Hairpin assembly-triggered cyclic activation of a DNA machine for label-free and ultrasensitive chemiluminescence detection of DNA
文献类型：期刊论文
| 作者 | Chen J(陈佳)1,2 ; Qiu HD(邱洪灯)1,2; Zhang ML(张明亮)1,2; Gu TN(顾桐年)1,2; Shao SJ(邵士俊)1,2; Huang Y(黄勇)3; Zhao SL(赵书林)3; Qiu HD(邱洪灯)
|
| 刊名 | Biosensors and Bioelectronics
 |
| 出版日期 | 2015 |
| 卷号 | 68页码:550-555 |
| 关键词 | P53 DNA Hairpin assembly DNA machine Label-free Chemiluminescence |
| ISSN号 | 0956-5663 |
| 通讯作者 | 邱洪灯 |
| 英文摘要 | DNA plays important regulatory roles in many life activities. Here, we have developed a novel label-free, ultrasensitive and specific chemiluminescence (CL) assay protocol for DNA detection based on hairpin assembly-triggered cyclic activation of a DNA machine. The system involves two hairpin structures, H1 and H2. Firstly, a target DNA binds with and opens the hairpin structure of H1. Then, H2 hybridizes with H1 and displaces the target DNA, which is used to trigger another new hybridization cycle between H1 and H2, leading to the generation of numerous H1–H2 complexes. The generated H1–H2 complexes are further activated with the help of polymerase and nicking enzyme, continuously yielding a large amount of G-riched DNA fragments. The G-riched DNA fragment products interact with hemin to form the activated HRP-mimicking DNA zymes that can catalyze the oxidation of luminal by H2O2 to produce strong CL signal resulting in an amplified sensing process. Our newly proposed homogeneous assay enables the quantitative measurement of p53 DNA (as a model) with a detection limit of 0.85 fM, which is at least 5 orders of magnitude lower than that of traditional unamplified homogeneous optical approaches. Moreover, this assay exhibits high discrimination ability even against a single base mismatch. In addition, this strategy is also capable of detecting p53 DNA in complex biological samples. The proposed sensing approach might hold a great promise for further applications in biomedical research and early clinical diagnosis. |
| 学科主题 | 分析化学与药物化学 |
| 收录类别 | SCI |
| 资助信息 | the “Hundred Talents Program” of the Chinese Academy of Science;the National Natural Science Foundation of China (Nos.21405163;21475142);the Foundation for Sci & Tech Research Project of Gansu Province (145RJYA271) |
| 语种 | 英语 |
| WOS记录号 | WOS:000351248300078 |
| 公开日期 | 2015-03-11 |
| 源URL | [http://210.77.64.217/handle/362003/7159]  |
| 专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
| 通讯作者 | Qiu HD(邱洪灯) |
| 作者单位 | 1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Guangxi Normal Univ, Coll Chem & Pharm, Key Lab Chem & Mol Engn Med Resources, Minist Educ China, Guilin 541004, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen J,Qiu HD,Zhang ML,et al. Hairpin assembly-triggered cyclic activation of a DNA machine for label-free and ultrasensitive chemiluminescence detection of DNA[J]. Biosensors and Bioelectronics,2015,68:550-555. |
| APA | Chen J.,Qiu HD.,Zhang ML.,Gu TN.,Shao SJ.,...&邱洪灯.(2015).Hairpin assembly-triggered cyclic activation of a DNA machine for label-free and ultrasensitive chemiluminescence detection of DNA.Biosensors and Bioelectronics,68,550-555. |
| MLA | Chen J,et al."Hairpin assembly-triggered cyclic activation of a DNA machine for label-free and ultrasensitive chemiluminescence detection of DNA".Biosensors and Bioelectronics 68(2015):550-555. |
入库方式： OAI收割
来源：兰州化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。