Kinetic analysis of the weak affinity interaction between tris and lysozyme
文献类型：期刊论文
| 作者 | Kang TF(康腾飞) ; Hao WX(郝文欣); Niu Y(牛宇); Luo ZR(罗子人); Jin G(靳刚)
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| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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| 出版日期 | 2015-02-20 |
| 卷号 | 457期号:4页码:659-663 |
| 通讯作者邮箱 | gajin@imech.ac.cn |
| 关键词 | Kinetic analysis Weak affinity interaction TIRIE biosensor Pseudo-first-order interaction kinetics Association rate constant |
| ISSN号 | 0006-291X |
| 产权排序 | [Kang, Tengfei; Hao, Wenxin; Niu, Yu; Luo, Ziren; Jin, Gang] Chinese Acad Sci, Inst Mech, NML, Beijing 100190, Peoples R China; [Kang, Tengfei; Hao, Wenxin] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 通讯作者 | Jin, G (reprint author), Chinese Acad Sci, Inst Mech, NML, 15 Bei Si Huan West Rd, Beijing 100190, Peoples R China. |
| 中文摘要 | The biosensor based on total internal reflection imaging ellipsometry (TIRIE), regarded as an automotive real-time research approach for biomolecular interaction, is introduced to analyze the kinetic process of the weak interaction between tris and lysozyme. The experiment is performed by delivering lysozyme solution diluted to different concentrations to the biosensor substrate interface immobilized with tris. By applying pseudo-first-order interaction kinetics model, we are able to obtain the kinetic parameters from fitting experimental data. The calculated association rate constant and dissociation rate constant of tris and lysozyme interaction are in 10(-2) mol(-1) s(-1) and 10(3) s(-1) magnitude, respectively. To further improve TIRIE's ability for kinetically characterizing biomolecular interaction, a theoretical method to deduce associate rate constant before experiment is proposed. (C) 2015 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 分类号 | Q3 |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | REFLECTION IMAGING ELLIPSOMETRY ; BIOMOLECULAR INTERACTIONS ; PROTEIN INTERACTIONS ; BIOSENSOR ; ADSORPTION ; MODEL |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1016/j.bbrc.2015.01.044 |
| 语种 | 英语 |
| WOS记录号 | WOS:000350838800028 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/49926]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Kang TF,Hao WX,Niu Y,et al. Kinetic analysis of the weak affinity interaction between tris and lysozyme[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,457(4):659-663. |
| APA | Kang TF,Hao WX,Niu Y,Luo ZR,&Jin G.(2015).Kinetic analysis of the weak affinity interaction between tris and lysozyme.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,457(4),659-663. |
| MLA | Kang TF,et al."Kinetic analysis of the weak affinity interaction between tris and lysozyme".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 457.4(2015):659-663. |
入库方式： OAI收割
来源：力学研究所
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