Visual detection of telomerase activity with a tunable dynamic range by using a gold nanoparticle probe-based hybridization protection strategy
文献类型：期刊论文
| 作者 | Wang, Jiasi ; Wu, Li ; Ren, Jinsong ; Qu,Xiaogang |
| 刊名 | nanoscale
 |
| 出版日期 | 2014 |
| 卷号 | 6期号:3页码:1661-1666 |
| 关键词 | AMPLIFICATION PROTOCOL ASSAY G-QUADRUPLEX LIGANDS COLORIMETRIC DETECTION SMALL MOLECULES DNA CANCER INHIBITION TARGET PRIMER IONS |
| ISSN号 | 2040-3364 |
| 通讯作者 | qu,xg |
| 中文摘要 | we developed a novel telomere complementary (tc) oligonucleotide modified aunp probe (tc-aunps) for colorimetric analysis of telomerase activity. the mechanism of this method is that the telomerase reaction products (trp), which can hybridize with the tc-aunps, are able to protect the aunps from the aggregation induced by salt. it is demonstrated that the colorimetric method enabled the analysis of the telomerase activity in 1000 hela cells with the naked eye, and down to 100 hela cells with the aid of uv-vis spectroscopy. this strategy is not only convenient and sensitive, but also has a tunable dynamic range. the platform is also applicable for the initial screening of a telomerase inhibitor to discover new anticancer drugs. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000330041400057 |
| 公开日期 | 2015-03-26 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/50701]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang, Jiasi,Wu, Li,Ren, Jinsong,et al. Visual detection of telomerase activity with a tunable dynamic range by using a gold nanoparticle probe-based hybridization protection strategy[J]. nanoscale,2014,6(3):1661-1666. |
| APA | Wang, Jiasi,Wu, Li,Ren, Jinsong,&Qu,Xiaogang.(2014).Visual detection of telomerase activity with a tunable dynamic range by using a gold nanoparticle probe-based hybridization protection strategy.nanoscale,6(3),1661-1666. |
| MLA | Wang, Jiasi,et al."Visual detection of telomerase activity with a tunable dynamic range by using a gold nanoparticle probe-based hybridization protection strategy".nanoscale 6.3(2014):1661-1666. |
入库方式： OAI收割
来源：长春应用化学研究所
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