Assaying multiple restriction endonucleases functionalities and inhibitions on DNA microarray with multifunctional gold nanoparticle probes
文献类型：期刊论文
| 作者 | Ma, Lan ; Zhu, Zhijun ; Li, Tao ; Wang,Zhenxin |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2014 |
| 卷号 | 52期号:1页码:118-123 |
| 关键词 | SENSITIVE DETECTION CLEAVAGE BINDING ENZYME ECORI IDENTIFICATION SPECIFICITY RECOGNITION COMPLEXES PLATFORM |
| 通讯作者 | wang,zx |
| 英文摘要 | herein, a double-stranded (ds) dna microarray-based resonance light scattering (rls) assay with multifunctional gold nanoparticle (gnp) probes has been developed for studying restriction endonuclease functionality and inhibition. because of decreasing significantly melting temperature, the enzyme-cleaved dsdnas easily unwind to form single-stranded (ss) dnas. the ssdnas are hybridized with multiplex complementary ssdnas functionalized gnp probes followed by silver enhancement and rls detection. three restriction endonucleases (ecori, bamhi and ecorv) and three potential inhibitors (doxorubicin hydrochloride (dox), ethidium bromide (eb) and an ecori-derived helical peptide (alpha 4)) were selected to demonstrate capability of the assay. enzyme activities of restriction endonucleases are detected simultaneously with high specificity down to the limits of 2.0 x 10(-2) u/ml for ecori, 1.1 x 10(-2) u/ml for bamhi and 1.6 x 10(-2) u/ml for ecorv, respectively. more importantly, the inhibitory potencies of three inhibitors are showed quantitatively, indicating that our approach has great promise for high-throughput screening of restriction endonuclease inhibitors. (c) 2013 elsevier b.v. all rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000328095400018 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/51052]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Ma, Lan,Zhu, Zhijun,Li, Tao,et al. Assaying multiple restriction endonucleases functionalities and inhibitions on DNA microarray with multifunctional gold nanoparticle probes[J]. biosensors & bioelectronics,2014,52(1):118-123. |
| APA | Ma, Lan,Zhu, Zhijun,Li, Tao,&Wang,Zhenxin.(2014).Assaying multiple restriction endonucleases functionalities and inhibitions on DNA microarray with multifunctional gold nanoparticle probes.biosensors & bioelectronics,52(1),118-123. |
| MLA | Ma, Lan,et al."Assaying multiple restriction endonucleases functionalities and inhibitions on DNA microarray with multifunctional gold nanoparticle probes".biosensors & bioelectronics 52.1(2014):118-123. |
入库方式： OAI收割
来源：长春应用化学研究所
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