Label-free detection of kanamycin using aptamer-based cantilever array sensor
文献类型：期刊论文
| 作者 | Bai, Xiaojing ; Hou, Hui ; Zhang, Bailin ; Tang,Jilin |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2014 |
| 卷号 | 56页码:112-116 |
| 关键词 | BIOSENSORS MICROCANTILEVERS NANOMECHANICS RECOGNITION MOLECULES SELECTION LIGANDS |
| 通讯作者 | zhang,bl |
| 英文摘要 | a label-free detection method of kanamycin using aptamer-based cantilever array sensor was developed. the cantilever array was composed of sensing cantilevers and reference cantilevers. this configuration allowed direct detection of individual cantilever deflections and subsequent determination of differential deflection of sensing/reference cantilever pair. the sensing cantilevers were functionalized with kanamycin aptamer, which was used as receptor molecules while the reference cantilevers were modified with 6-mercapto-1-hexanol (mch) to eliminate the influence of environmental disturbances. the kanamycin-aptamer interaction induced a change in cantilever surface stress, which caused a differential deflection between the sensing and reference cantilever pair. the surface stress change was linear with kanamycin concentration over the range of 100 mu m-10 mm with a correlation coefficient of 0.995. a detection limit of 50 mu m was obtained, at a signal-to-noise ratio of 3. the sensor also showed good selectivity against other antibiotics such as neomycin, ribostamycin and chloramphenicol. the facile method for kanamycin detection may have great potential for investigating more other molecules. (c) 2014 elsevier b.v. all rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000333781000018 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/51057]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Bai, Xiaojing,Hou, Hui,Zhang, Bailin,et al. Label-free detection of kanamycin using aptamer-based cantilever array sensor[J]. biosensors & bioelectronics,2014,56:112-116. |
| APA | Bai, Xiaojing,Hou, Hui,Zhang, Bailin,&Tang,Jilin.(2014).Label-free detection of kanamycin using aptamer-based cantilever array sensor.biosensors & bioelectronics,56,112-116. |
| MLA | Bai, Xiaojing,et al."Label-free detection of kanamycin using aptamer-based cantilever array sensor".biosensors & bioelectronics 56(2014):112-116. |
入库方式： OAI收割
来源：长春应用化学研究所
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