Fabricating three-dimensional carbohydrate hydrogel microarray for lectin-mediated bacterium capturing
文献类型：期刊论文
| 作者 | Liu, Xia ; Lei, Zhen ; Liu, Fuyao ; Liu,Dianjun ; Wang,Zhenxin |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2014 |
| 卷号 | 58期号:10页码:92-100 |
| 关键词 | ENTEROHEMORRHAGIC ESCHERICHIA-COLI SALMONELLA-TYPHIMURIUM LISTERIA-MONOCYTOGENES ANTIBODY MICROARRAY MASS-SPECTROMETRY TYPE-1 FIMBRIAE PROTEIN RECOGNITION BIOSENSOR PATHOGENS |
| 通讯作者 | wang,zx |
| 英文摘要 | herein, a three-dimensional carbohydrate modified polyacrylamide hydrogel microarray (3d carbohydrate hydrogel microarray) has been fabricated and employed as micro-reactor for capturing escherichia coli (e. coli) by multivalent binding of concanavalin a (con a) with o-antigen on the cellular surface of e. coli and immobilized monosaccharides on hydrogel spot, and the interactions of type 1 fimbriae of e. coli with immobilized monosaccharides. because of the transparent performance of polyacrylamide hydrogel, the captured e. coli can be directly observed by a conventional microscope under a bright-field mode. the experimental result demonstrates that a-d-mannopyranoside (man-a) modified hydrogel surface shows high efficiency of e. coli capturing. the 3d man-a hydrogel microarray-based assay shows reasonable low detection limit (1.0 x 10(4) cells/ml) and large dynamic range (1.0 x 10(5) to 1.0 x 10(9)cells/ml) for detecting e. coli. in addition, bacterial adhesion inhibition assay has been demonstrated by the interactions of e. coli with ten saccharides, and satisfactory results have been obtained. (c) 2014 elsevier b.v. all rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000336109900015 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/51058]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu, Xia,Lei, Zhen,Liu, Fuyao,et al. Fabricating three-dimensional carbohydrate hydrogel microarray for lectin-mediated bacterium capturing[J]. biosensors & bioelectronics,2014,58(10):92-100. |
| APA | Liu, Xia,Lei, Zhen,Liu, Fuyao,Liu,Dianjun,&Wang,Zhenxin.(2014).Fabricating three-dimensional carbohydrate hydrogel microarray for lectin-mediated bacterium capturing.biosensors & bioelectronics,58(10),92-100. |
| MLA | Liu, Xia,et al."Fabricating three-dimensional carbohydrate hydrogel microarray for lectin-mediated bacterium capturing".biosensors & bioelectronics 58.10(2014):92-100. |
入库方式： OAI收割
来源：长春应用化学研究所
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