Reversible detection of vancomycin using peptide-functionalized cantilever array sensor
文献类型：期刊论文
| 作者 | Bai, Xiaojing ; Lu, Baoping ; Chen, Xuejuan |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2014 |
| 卷号 | 62期号:1页码:145-150 |
| 关键词 | FLUORESCENCE POLARIZATION IMMUNOASSAY PERFORMANCE LIQUID-CHROMATOGRAPHY GLYCOPEPTIDE ANTIBIOTICS NANOMECHANICAL DETECTION BINDING ASSAY DRUG MICROCANTILEVER RECOGNITION MECHANISM |
| 通讯作者 | zhang,bl |
| 英文摘要 | a reversible detection method for vancomycin was developed utilizing the cantilever array sensor functionalized by a designed peptide consisting of a cysteine (cys-), a space linker (-gly-gly-gly-gly-) and a molecular recognition ligand (-l-lys-d-ala-d-ala). it was found that the peptide space linker was necessary and important for the response of the cantilever array sensor. the sensing cantilevers in the array were functionalized with the peptide while the reference cantilevers were modified by 6-mercapto-1-hexanol (mch) to eliminate the influence of environmental disturbances. the binding between vancomycin and the peptide induced a change of surface stresses in the sensing cantilevers resulting in a differential deflection between the sensing and reference cantilevers. the reciprocal of the differential deflection is linear with the reciprocal of vancomycin concentration within the range of 2 mu m to 100 mu m (r=0.993) at a detection limit of 0.2 mu m (s/n=3). the reversible detection can be realized just by regenerating the sensing cantilevers with running buffer solution. other antibiotics such as doxycycline, streptomycin, and kanamycin have negligible effect on the response of the sensor. the sensor can also be utilized for reversible detection of vancomycin in serum background, which clearly indicates the potential of the sensor for vancomycin detection in real biological samples. (c) 2014 elsevier b.v. all rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000340334800024 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/51063]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Bai, Xiaojing,Lu, Baoping,Chen, Xuejuan. Reversible detection of vancomycin using peptide-functionalized cantilever array sensor[J]. biosensors & bioelectronics,2014,62(1):145-150. |
| APA | Bai, Xiaojing,Lu, Baoping,&Chen, Xuejuan.(2014).Reversible detection of vancomycin using peptide-functionalized cantilever array sensor.biosensors & bioelectronics,62(1),145-150. |
| MLA | Bai, Xiaojing,et al."Reversible detection of vancomycin using peptide-functionalized cantilever array sensor".biosensors & bioelectronics 62.1(2014):145-150. |
入库方式： OAI收割
来源：长春应用化学研究所
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