Quantitative analyses of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) large-subunit genes (cbbL) in typical paddy soils
文献类型：期刊论文
| 作者 | Xiao, Ke-Qing ; Bao, Peng ; Bao, Qiong-Li ; Jia, Yan ; Huang, Fu-Yi ; Su, Jian-Qiang ; Zhu, Yong-Guan |
| 刊名 | FEMS MICROBIOLOGY ECOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 87期号:1页码:89-101 |
| 关键词 | RubisCO cbbL CO2 fixation paddy soil |
| ISSN号 | 0168-6496 |
| 中文摘要 | The Calvin cycle is known to be the major pathway for CO2 fixation, but our current understanding of its occurrence and importance in paddy soils is poor. In this study, the diversity of three ribulose-1,5-bisphosphate carboxylase/oxygenase large-subunit genes (cbbLG, cbbLR, cbbM) was investigated by clone library, T-RFLP, qPCR, and enzyme assay in five paddy soils in China. The cbbLG sequences revealed a relatively low level of diversity and were mostly related to the sequences of species from Thiobacillus. In contrast, highly diverse cbbLR and cbbM sequences were dispersed on the phylogenetic trees, and most of them were distantly related to known sequences, even forming separate clusters. Abundances of three cbbL genes ranged from 10(6) to 10(9)copiesg(-1) soil, and cbbLR outnumbered cbbM and cbbLG in all soil samples, indicating that cbbLR may play a more important role than other two cbbL genes. Soil properties significantly influenced cbbL diversity in five paddy soils, of which clay content, C/N ratio, CEC, pH, and SOC correlated well with variations in microbial composition and abundance. In summary, this study provided a comparison of three cbbL genes, advancing our understanding of their role in carbon sequestration and nutrient turnover in the paddy soil. |
| WOS记录号 | WOS:000329170100009 |
| 公开日期 | 2015-03-23 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9214]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 生态环境研究中心_土壤环境科学实验室 |
| 推荐引用方式 GB/T 7714 | Xiao, Ke-Qing,Bao, Peng,Bao, Qiong-Li,et al. Quantitative analyses of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) large-subunit genes (cbbL) in typical paddy soils[J]. FEMS MICROBIOLOGY ECOLOGY,2014,87(1):89-101. |
| APA | Xiao, Ke-Qing.,Bao, Peng.,Bao, Qiong-Li.,Jia, Yan.,Huang, Fu-Yi.,...&Zhu, Yong-Guan.(2014).Quantitative analyses of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) large-subunit genes (cbbL) in typical paddy soils.FEMS MICROBIOLOGY ECOLOGY,87(1),89-101. |
| MLA | Xiao, Ke-Qing,et al."Quantitative analyses of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) large-subunit genes (cbbL) in typical paddy soils".FEMS MICROBIOLOGY ECOLOGY 87.1(2014):89-101. |
入库方式： OAI收割
来源：生态环境研究中心
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