A sensitive fluorescence anisotropy method for detection of lead (II) ion by a G-quadruplex-inducible DNA aptamer
文献类型：期刊论文
| 作者 | Zhang, Dapeng
|
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2014 |
| 卷号 | 812期号:1页码:161-167 |
| 关键词 | Lead ion G-quadruplex Fluorescence anisotropy Thrombin binding aptamer |
| ISSN号 | 0003-2670 |
| 中文摘要 | Sensitive and selective detection of Pb2+ is of great importance to both human health and environmental protection. Here we propose a novel fluorescence anisotropy (FA) approach for sensing Pb2+ in homogeneous solution by a G-rich thrombin binding aptamer (TBA). The TBA labeled with 6-carboxytetramethylrhodamine (TMR) at the seventh thymine nucleotide was used as a fluorescent probe for signaling Pb2+. It was found that the aptamer probe had a high FA in the absence of Pb2+. This is because the rotation of TMR is restricted by intramolecular interaction with the adjacent guanine bases, which results in photoinduced electron transfer (PET). When the aptamer probe binds to Pb2+ to form G-quadruplex, the intramolecular interaction should be eliminated, resulting in faster rotation of the fluorophore TMR in solution. Therefore, FA of aptamer probe is expected to decrease significantly upon binding to Pb2+. Indeed, we observed a decrease in FA of aptamer probe upon Pb2+ binding. Circular dichroism, fluorescence spectra, and fluorescence lifetime measurement were used to verify the reliability and reasonability of the sensing mechanism. By monitoring the FA change of the aptamer probe, we were able to real-time detect binding between the TBA probe and Pb2+. Moreover, the aptamer probe was exploited as a recognition element for quantification of Pb2+ in homogeneous solution. The change in FA showed a linear response to Pb2+ from 10 nM to 2.0 mu M, with 1.0 nM limit of detection. In addition, this sensing system exhibited good selectivity for Pb2+ over other metal ions. The method is simple, quick and inherits the advantages of aptamer and FA. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS记录号 | WOS:000330581200022 |
| 公开日期 | 2015-03-24 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9305]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, Dapeng. A sensitive fluorescence anisotropy method for detection of lead (II) ion by a G-quadruplex-inducible DNA aptamer[J]. ANALYTICA CHIMICA ACTA,2014,812(1):161-167. |
| APA | Zhang, Dapeng.(2014).A sensitive fluorescence anisotropy method for detection of lead (II) ion by a G-quadruplex-inducible DNA aptamer.ANALYTICA CHIMICA ACTA,812(1),161-167. |
| MLA | Zhang, Dapeng."A sensitive fluorescence anisotropy method for detection of lead (II) ion by a G-quadruplex-inducible DNA aptamer".ANALYTICA CHIMICA ACTA 812.1(2014):161-167. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。