Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry: A novel approach for clinical monitoring and doping control
文献类型：期刊论文
| 作者 | Guo, Feng ; Shao, Jing ; Liu, Qian ; Shi, Jian-bo ; Jiang, Gui-bin |
| 刊名 | TALANTA
 |
| 出版日期 | 2014 |
| 卷号 | 125期号:1页码:432-438 |
| 关键词 | Online turboflow solid-phase extraction Anabolic androgenic steroids Urine Clinical monitoring Doping control |
| ISSN号 | 0039-9140 |
| 中文摘要 | A novel method for automated and sensitive analysis of testosterone, androstenedione, methyltestosterone and methenolone in urine samples by online turbulent flow solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry was developed. The optimization and validation of the method were discussed in detail. The Turboflow C18-P SPE column showed the best extraction efficiency for all the analytes. Nanogram per liter (ng/L) level of MS could be determined directly and the limits of quantification (LOQs) were 0.01 ng/mL, which were much lower than normally concerned concentrations for these typical anabolic androgenic steroids (AAS) (0.1 ng/mL). The linearity range was from the LOQ to 100 ng/mL for each compound, with the coefficients of determination (r(2)) ranging from 0.9990 to 0.9999. The intraday and interday relative standard deviations (RSDs) ranged from 1.1% to 14.5% (n=5). The proposed method was successfully applied to the analysis of urine samples collected from 24 male athletes and 15 patients of prostate cancer. The proposed method provides an alternative practical way to rapidly determine MS in urine samples, especially for clinical monitoring and doping control. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS记录号 | WOS:000336872200062 |
| 公开日期 | 2015-03-24 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9319]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Guo, Feng,Shao, Jing,Liu, Qian,et al. Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry: A novel approach for clinical monitoring and doping control[J]. TALANTA,2014,125(1):432-438. |
| APA | Guo, Feng,Shao, Jing,Liu, Qian,Shi, Jian-bo,&Jiang, Gui-bin.(2014).Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry: A novel approach for clinical monitoring and doping control.TALANTA,125(1),432-438. |
| MLA | Guo, Feng,et al."Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry: A novel approach for clinical monitoring and doping control".TALANTA 125.1(2014):432-438. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。