Cellular target recognition of perfluoroalkyl acids: In vitro evaluation of inhibitory effects on lysine decarboxylase
文献类型：期刊论文
| 作者 | Wang, Sufang ; Lv, Qiyan ; Yang, Yu ; Guo, Liang-Hong ; Wan, Bin ; Zhao, Lixia |
| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
 |
| 出版日期 | 2014 |
| 卷号 | 496期号:1页码:381-388 |
| 关键词 | Perfluoroalkyl acid Lysine decarboxylase Inhibition Cellular target |
| ISSN号 | 0048-9697 |
| 中文摘要 | Perfluoroalkyl adds (PFAAs) have been shown to bind with hepatic peroxisome proliferator receptor a, estrogen receptors and human serum albumin and subsequently cause some toxic effects. Lysine decarboxylase (LDC) plays an important role in cell growth and developmental processes. In this study, the inhibitory effect of 16 PFAAs, including 13 perfluorinated carboxylic acids (PFCAs) and 3 perfluorinated sulfonic acids (PFSAs), on lysine decarboxylase (LDC) activity was investigated. The inhibition constants obtained in fluorescence enzyme assays fall in the range of 2.960 mu pM to 290.8 mu M for targeted PFCAs, and 41.22 mu M to 67.44 mu M for targeted PFSAs. The inhibitory effect of PFCAs increased significantly with carbon chain (7-18 carbons), whereas the short chain PFCAs (less than 7 carbons) did not show any effect. Circular dichroism results showed that PFAA binding induced significant protein secondary structural changes. Molecular docking revealed that the inhibitory effect could be rationalized well by the cleft binding mode as well as the size, substituent group and hydrophobic characteristics of the PFAAs. At non-cytotoxic concentrations, three selected PFAAs inhibited LDC activity in HepG2 cells, and subsequently resulted in the decreased cadaverine level in the exposed cells, suggesting that LDC may be a possible target of PFAAs for their in vivo toxic effects. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS记录号 | WOS:000342245600044 |
| 公开日期 | 2015-03-24 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9325]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Sufang,Lv, Qiyan,Yang, Yu,et al. Cellular target recognition of perfluoroalkyl acids: In vitro evaluation of inhibitory effects on lysine decarboxylase[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2014,496(1):381-388. |
| APA | Wang, Sufang,Lv, Qiyan,Yang, Yu,Guo, Liang-Hong,Wan, Bin,&Zhao, Lixia.(2014).Cellular target recognition of perfluoroalkyl acids: In vitro evaluation of inhibitory effects on lysine decarboxylase.SCIENCE OF THE TOTAL ENVIRONMENT,496(1),381-388. |
| MLA | Wang, Sufang,et al."Cellular target recognition of perfluoroalkyl acids: In vitro evaluation of inhibitory effects on lysine decarboxylase".SCIENCE OF THE TOTAL ENVIRONMENT 496.1(2014):381-388. |
入库方式： OAI收割
来源：生态环境研究中心
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