Co-exposure of Carboxyl-Functionalized Single-Walled Carbon Nanotubes and 17 alpha-Ethinylestradiol in Cultured Cells: Effects on Bioactivity and Cytotoxicity
文献类型：期刊论文
| 作者 | Song, Maoyong ; Wang, Fengbang ; Zeng, Luzhe ; Yin, Junfa ; Wang, Hailin ; Jiang, Guibin |
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 48期号:23页码:13978-13984 |
| ISSN号 | 0013-936X |
| 中文摘要 | 17 alpha-Ethinylestradiol (EE2) is the representative of environmental estrogens. Although EE2 can interact with some engineered nanoparticles (NPs), little is known about the bioactivity of NP-associated EE2 in organisms. In this study, we investigated the combined effects of the co-exposed carboxyl-functionalized single-walled carbon nanotubes (cf-SWCNTs) and EE2 in the human breast adenocarcinoma cell line (MCF-7 cells), focusing on the cytotoxicity and bioactivity. There were no significant differences in mitochondrial activity, membrane damage, and cell apoptosis when exposed to cf-SWCNTs with and without adsorbed EE2. However, the bioactivity of adsorbed EE2 on cf-SWCNTs was significantly inhibited. The calculated effective concentration of EE2 in cultured cells showed that less than 0.2% of the total adsorbed EE2 was released, indicating that most EE2 was retained on the cf-SWCNTs during cellular exposure. Furthermore, there were no obvious changes in the bioactivity of adsorbed EE2 in the culture medium containing 520% fetal bovine serum (FBS), even up to 10 days of incubation, indicating that the adsorbed EE2 on cf-SWCNTs is highly stable in the cell culture medium. These results mark a promising possibility for EE2 to be adsorbed by cf-SWCNTs in environmentally relevant settings and thereby influenced its toxicity and biological fate. This is also tempting for future studies involving risk assessment ways for association between NPs and contaminants in the environment. |
| WOS记录号 | WOS:000345803800051 |
| 公开日期 | 2015-03-24 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9329]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Song, Maoyong,Wang, Fengbang,Zeng, Luzhe,et al. Co-exposure of Carboxyl-Functionalized Single-Walled Carbon Nanotubes and 17 alpha-Ethinylestradiol in Cultured Cells: Effects on Bioactivity and Cytotoxicity[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2014,48(23):13978-13984. |
| APA | Song, Maoyong,Wang, Fengbang,Zeng, Luzhe,Yin, Junfa,Wang, Hailin,&Jiang, Guibin.(2014).Co-exposure of Carboxyl-Functionalized Single-Walled Carbon Nanotubes and 17 alpha-Ethinylestradiol in Cultured Cells: Effects on Bioactivity and Cytotoxicity.ENVIRONMENTAL SCIENCE & TECHNOLOGY,48(23),13978-13984. |
| MLA | Song, Maoyong,et al."Co-exposure of Carboxyl-Functionalized Single-Walled Carbon Nanotubes and 17 alpha-Ethinylestradiol in Cultured Cells: Effects on Bioactivity and Cytotoxicity".ENVIRONMENTAL SCIENCE & TECHNOLOGY 48.23(2014):13978-13984. |
入库方式： OAI收割
来源：生态环境研究中心
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