Environmental (anti-)androgenic chemicals affect germinal vesicle breakdown (GVBD) of Xenopus laevis oocytes in vitro
文献类型：期刊论文
| 作者 | Cao, Shan ; Xu, Wei ; Lou, Qin-Qin ; Zhang, Yin-Feng ; Zhao, Ya-Xian ; Wei, Wu-Ji ; Qin, Zhan-Fen |
| 刊名 | TOXICOLOGY IN VITRO
 |
| 出版日期 | 2014 |
| 卷号 | 28期号:3页码:426-431 |
| 关键词 | Xenopus laevis Germinal vesicle breakdown Testosterone Trenbolone Nandrolone Androgen receptor antagonist |
| ISSN号 | 0887-2333 |
| 中文摘要 | Progesterone-induced germinal vesicle breakdown (GVBD) of Xenopus oocytes in vitro was used to study endocrine disrupting activity of chemicals in previous studies. In this study, we investigated for the first time effects of environmental androgens on oocyte maturation and effects of anti-androgens on androgen-induced oocyte maturation, using Xenopus GVBD in vitro. Trenbolone and nandrolone, two environmental androgens, were found to induce Xenopus GVBD at low concentrations. The potential of trenbolone to induce GVBD was approximately 100-fold lower than that of testosterone, while nandrolone had a several-fold lower potential than testosterone. Our findings have aroused new concerns for effects of environmental androgens on amphibian oocyte maturation at environmentally relevant concentrations, and suggested that Xenopus GVBD can be used to test androgenic activity of suspicious environmental androgens. Androgen receptor (AR) antagonist flutamide at 10 mu M only exhibited a weakly inhibitory effect on androgen-induced GVBD, while another known AR antagonist vinclozolin had no effect even at high concentrations. The results show that Xeno pus GVBD is not sensitive to AR-mediated environmental anti-androgens. In contrast to flutamide and vinclozolin, methoxychlor (a weaker AR antagonist) inhibited dramatically androgen-induced GVBD, suggesting that androgen-induced Xenopus GVBD can be used to study non-AR-mediated effects of chemicals on oocyte maturation. (C) 2013 Elsevier Ltd. All rights reserved. |
| WOS记录号 | WOS:000332053800012 |
| 公开日期 | 2015-03-24 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9362]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Cao, Shan,Xu, Wei,Lou, Qin-Qin,et al. Environmental (anti-)androgenic chemicals affect germinal vesicle breakdown (GVBD) of Xenopus laevis oocytes in vitro[J]. TOXICOLOGY IN VITRO,2014,28(3):426-431. |
| APA | Cao, Shan.,Xu, Wei.,Lou, Qin-Qin.,Zhang, Yin-Feng.,Zhao, Ya-Xian.,...&Qin, Zhan-Fen.(2014).Environmental (anti-)androgenic chemicals affect germinal vesicle breakdown (GVBD) of Xenopus laevis oocytes in vitro.TOXICOLOGY IN VITRO,28(3),426-431. |
| MLA | Cao, Shan,et al."Environmental (anti-)androgenic chemicals affect germinal vesicle breakdown (GVBD) of Xenopus laevis oocytes in vitro".TOXICOLOGY IN VITRO 28.3(2014):426-431. |
入库方式： OAI收割
来源：生态环境研究中心
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