Investigation of noncovalent interactions between hydroxylated polybrominated diphenyl ethers and bovine serum albumin using electrospray ionization-ion mobility-mass spectrometry
文献类型：期刊论文
| 作者 | Ma, Qiang ; Bai, Hua ; Wang, Chao ; Xi, Guang-Cheng ; Zhang, Qing ; Meng, Xian-Shuang ; Chen, Yun-Xia ; Li, Jing-Rui ; Ma, Hui-Juan ; Guo, Liang-Hong |
| 刊名 | INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
 |
| 出版日期 | 2014 |
| 卷号 | 357期号:1页码:34-44 |
| 关键词 | Hydroxylated polybrominated diphenyl ethers Bovine serum albumin Noncovalent interaction Electrospray ionization Ion mobility-mass spectrometry |
| ISSN号 | 1387-3806 |
| 中文摘要 | The noncovalent interactions of 24 diversely structured hydroxylated polybrominated diphenyl ethers (OH-PBDEs), ranging from hydroxylated monobromodiphenyl ether (OH-monoBDE) to hydroxylated octabromodiphenyl ether (OH-octaBDE), with bovine serum albumin (BSA) have been examined by employing an electrospray ionization source fitted on a quadrupole time-of-flight hybrid mass spectrometer equipped with a traveling wave ion mobility cell. The mass spectrometric parameters were finely optimized to favor the observation of noncovalent complexes. The experimental data confirm that due to the close structural resemblance with thyroid hormone thyroxine, some OH-PBDEs have been shown to conjugate with BSA. It is found that BSA preferentially interacts with one OH-octaBDE (4'-OH-BDE-201) and two hydroxylated heptabromodiphenyl ether (OH-heptaBDE) isomers (4-OH-BDE-187 and 6-OH-BDE-180) with 1:1 and 2:1 binding stoichiometries. The state of bound OH-PBDEs is inferred to be as both neutral and anionic species. The dissociation constants of corresponding noncovalent complexes are calculated by titration curve data fitting. Investigation into various OH-PBDE analogs demonstrates that both the degree of bromination and the positions of hydroxylation and bromine moieties may exert influence on the binding of OH-PBDEs to BSA. The possible conformational changes in BSA induced upon the binding of OH-PBDEs were explored by use of ion mobility-mass spectrometry (IM-MS) to understand the details of the interactions in structural aspects. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS记录号 | WOS:000329532700004 |
| 公开日期 | 2015-03-24 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9393]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Ma, Qiang,Bai, Hua,Wang, Chao,et al. Investigation of noncovalent interactions between hydroxylated polybrominated diphenyl ethers and bovine serum albumin using electrospray ionization-ion mobility-mass spectrometry[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2014,357(1):34-44. |
| APA | Ma, Qiang.,Bai, Hua.,Wang, Chao.,Xi, Guang-Cheng.,Zhang, Qing.,...&Guo, Liang-Hong.(2014).Investigation of noncovalent interactions between hydroxylated polybrominated diphenyl ethers and bovine serum albumin using electrospray ionization-ion mobility-mass spectrometry.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,357(1),34-44. |
| MLA | Ma, Qiang,et al."Investigation of noncovalent interactions between hydroxylated polybrominated diphenyl ethers and bovine serum albumin using electrospray ionization-ion mobility-mass spectrometry".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY 357.1(2014):34-44. |
入库方式： OAI收割
来源：生态环境研究中心
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