Molecular characterization and developmental expression patterns of thyroid hormone receptors (TRs) and their responsiveness to TR agonist and antagonist in Rana nigromaculata
文献类型：期刊论文
| 作者 | Lou, Qinqin ; Zhang, Yinfeng ; Ren, Dongkai ; Xu, Haiming ; Zhao, Yaxian ; Qin, Zhanfen ; Wei, Wuji |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 出版日期 | 2014 |
| 卷号 | 26期号:10页码:2084-2094 |
| 关键词 | Rana nigromaculata Thyroid hormone receptor Thyroid hormone signaling Developmental expression pattern Responsiveness |
| ISSN号 | 1001-0742 |
| 中文摘要 | Considering some advantages of Rana nigromaculata as an experimental species, we propose that this species, like Xenopus laevis, could be used to assay thyroid hormone (TH) signaling disrupting actions. To validate the utilizability of R. nigromaculata, we investigated the responsiveness of R. nigromaculata to a TH receptor (TR) agonist (T3) and antagonist (amiodarone) by analyzing expression, based on characterizing TR cDNA and developmental expression patterns. With high levels of identity with the corresponding genes in X. laevis, both TR alpha and TR beta in R. nigromaculata exhibited roughly similar developmental expression patterns to those of X. laevis, in spite of some species-specific differences. Both TR alpha and TR beta expression had greater changes in the liver and intestine than in the tail and brain during metamorphosis. T3 exposure for 2 days induced more dramatic increases of TR beta expression in stage 27 than in stage 34 tadpoles but not in stage 42 tadpoles, showing that the responsiveness of R. nigromaculata to TH decreased with development and disappeared at the onset of metamorphic climax. Corresponding to greater changes of TR beta expression in the liver and intestine than in the tail and brain during metamorphosis, the liver and intestine had higher responsiveness to exogenous T3 than the tail and brain. Amiodarone inhibited T3-induced TR beta expression. Our results show that R. nigromaculata can be used as a model species for assaying TH signaling disrupting actions by analyzing TR beta expression, and intestine tissues at stage 27 are ideal test materials due to high responsiveness and easy accessibility. (C) 2014 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. |
| WOS记录号 | WOS:000343780700016 |
| 公开日期 | 2015-03-24 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9403]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Lou, Qinqin,Zhang, Yinfeng,Ren, Dongkai,et al. Molecular characterization and developmental expression patterns of thyroid hormone receptors (TRs) and their responsiveness to TR agonist and antagonist in Rana nigromaculata[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2014,26(10):2084-2094. |
| APA | Lou, Qinqin.,Zhang, Yinfeng.,Ren, Dongkai.,Xu, Haiming.,Zhao, Yaxian.,...&Wei, Wuji.(2014).Molecular characterization and developmental expression patterns of thyroid hormone receptors (TRs) and their responsiveness to TR agonist and antagonist in Rana nigromaculata.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,26(10),2084-2094. |
| MLA | Lou, Qinqin,et al."Molecular characterization and developmental expression patterns of thyroid hormone receptors (TRs) and their responsiveness to TR agonist and antagonist in Rana nigromaculata".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 26.10(2014):2084-2094. |
入库方式： OAI收割
来源：生态环境研究中心
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