Basal and benzo[a]pyrene-induced expression profile of phase I and II enzymes and ABC transporter mRNA in the early life stage of Chinese rare minnows (Gobiocypris rarus)
文献类型：期刊论文
| 作者 | Yuan, Lilai ; Lv, Biping ; Zha, Jinmiao ; Wang, Weimin ; Wang, Zijian |
| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| 出版日期 | 2014 |
| 卷号 | 106期号:1页码:86-94 |
| 关键词 | Xenobiotic enzymes/transporters Embryo toxicity PAHs RT-PCR |
| ISSN号 | 0147-6513 |
| 中文摘要 | ATP-binding cassette (ABC) transporters together with phase I and II detoxification enzymes have been considered as included in a cellular detoxification system. Previous studies have highlighted the involvement of aryl hydrocarbon receptor (AHR) and Cyp1a in PAH-induced embryo toxicity. However, the response of other xenobiotic enzymes/transporters in PAH-mediated embryo toxicity is not fully characterized. In the present study, rare minnow embryos were exposed to 10 and 100 mu g/L BaP within 4 h post-fertilization (hpf) up to 168 hpf. RNA was extracted at 24, 48, 96, and 168 hpf. The basal and BaP-induced expression of phase I enzyme genes (cyp1a, 1b1, and 1c1), phase II enzyme gene (gstm and ugt1a), and ABC transporter genes (abcb1, abcc1, abcc2, and abcg2) mRNA was determined using realtime PCR. Severe developmental defects (e.g., spinal deformities, pericardial and yolk-sac edema) were observed in the BaP treated groups. The basal expression showed that gstm was most strongly expressed, followed by abcb1, ugt1a, and abcc2, whereas cyp1a, 1b1, 1c1, and abcg2 showed weak expression. BaP significantly induced the mRNA expression of three CYP1s (cyp1a, 1b1, and 1c1) (p <0.05) and the ABC transporters (abcc1, abcc2, and abcg2) in a dose-dependent manner. However, the mRNA expression of Phase II enzymes (gstm, ugtla) for the BaP treatments showed no significant difference with that of the controls. Furthermore, distinct induced patterns of these genes were observed during different exposure periods. Simultaneous up-regulation of the cyp and ABC transporter gene transcripts suggests that a possible involvement and cooperation in the detoxification process could provide protection against the BaP toxicity of rare minnows at the early life stage. (C) 2014 Elsevier Inc. All rights reserved. |
| WOS记录号 | WOS:000337643800013 |
| 公开日期 | 2015-03-26 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9502]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yuan, Lilai,Lv, Biping,Zha, Jinmiao,et al. Basal and benzo[a]pyrene-induced expression profile of phase I and II enzymes and ABC transporter mRNA in the early life stage of Chinese rare minnows (Gobiocypris rarus)[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2014,106(1):86-94. |
| APA | Yuan, Lilai,Lv, Biping,Zha, Jinmiao,Wang, Weimin,&Wang, Zijian.(2014).Basal and benzo[a]pyrene-induced expression profile of phase I and II enzymes and ABC transporter mRNA in the early life stage of Chinese rare minnows (Gobiocypris rarus).ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,106(1),86-94. |
| MLA | Yuan, Lilai,et al."Basal and benzo[a]pyrene-induced expression profile of phase I and II enzymes and ABC transporter mRNA in the early life stage of Chinese rare minnows (Gobiocypris rarus)".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 106.1(2014):86-94. |
入库方式： OAI收割
来源：生态环境研究中心
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