Effects of dechlorane plus on the hepatic proteome of juvenile Chinese sturgeon (Acipenser sinensis)
文献类型：期刊论文
| 作者 | Liang, Xuefang ; Li, Wei ; Martyniuk, Christopher J. ; Zha, Jinmiao ; Wang, Zijian ; Cheng, Gang ; Giesy, John P. |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 148期号:1页码:83-91 |
| 关键词 | Dechlorane Plus Protein networks Toxicity pathways Juvenile Chinese sturgeon (Acipenser sinensis) |
| ISSN号 | 0166-445X |
| 中文摘要 | Dechlorane Plus (DP), an alternative to decabromodiphenyl ether (BDE-209), is a widely used polychlorinated flame retardant that is frequently detected in aquatic ecosystems. While the mechanisms of toxicity of BDE-209 have been well documented, less is known about the toxicity of DP. In this study, juvenile Chinese sturgeon (Acipenser sinensis) were treated with DP at doses of!, 10, and 100 mg/kg wet weight for 14 days via a single intraperitoneal injection (i.p.). After 14 days, liver proteomes of juvenile Chinese sturgeon were analyzed using two-dimensional electrophoresis (2-DE) coupled matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). A total of 39 protein spots were significantly altered in abundance (>2-fold) and of these proteins, 27 were successfully identified. Proteins related to the stress response that included heat shock cognate protein 70 and T-complex protein 1 were significantly increased and decreased in abundance, respectively. Moreover, Ras-related protein Rab-6B and GDP dissociation inhibitor 2, proteins that are involved in small G-protein signal cascades, were decreased in abundance 2- to 5-fold. Annexin A4, which is associated with Ca2+ signaling pathways, was also markedly decreased by 2-fold in the liver. Pathway analysis of differentially regulated proteins revealed that DP interfered with metabolism and was associated with proteins related to apoptosis and cell differentiation. Based upon protein responses, we suggest that DP has effects on the generalized stress response, small G-protein signal cascades, Ca2+ signaling pathway, and metabolic process, and may induce apoptosis in the liver. This study offers novel mechanistic insight into the protein responses induced in the liver with DP, an increasingly used and understudied flame retardant. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS记录号 | WOS:000333496200010 |
| 公开日期 | 2015-03-26 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9533]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Liang, Xuefang,Li, Wei,Martyniuk, Christopher J.,et al. Effects of dechlorane plus on the hepatic proteome of juvenile Chinese sturgeon (Acipenser sinensis)[J]. AQUATIC TOXICOLOGY,2014,148(1):83-91. |
| APA | Liang, Xuefang.,Li, Wei.,Martyniuk, Christopher J..,Zha, Jinmiao.,Wang, Zijian.,...&Giesy, John P..(2014).Effects of dechlorane plus on the hepatic proteome of juvenile Chinese sturgeon (Acipenser sinensis).AQUATIC TOXICOLOGY,148(1),83-91. |
| MLA | Liang, Xuefang,et al."Effects of dechlorane plus on the hepatic proteome of juvenile Chinese sturgeon (Acipenser sinensis)".AQUATIC TOXICOLOGY 148.1(2014):83-91. |
入库方式： OAI收割
来源：生态环境研究中心
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