Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye
文献类型：期刊论文
| 作者 | Yang, Bo-Yun ; Liu, Xiao-Lu ; Wei, Yu-Mei ; Wang, Jing-Qi ; He, Xiao-Qing ; Jin, Yi ; Wang, Zi-Jian |
| 刊名 | BMC MICROBIOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 14期号:1页码:- |
| 关键词 | Astrovirus Loop-mediated isothermal amplification Hydroxynaphthol blue |
| ISSN号 | 1471-2180 |
| 中文摘要 | Background: The aim of this paper was to develop a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for rapid, sensitive and inexpensive detection of astrovirus. Results: The detection limit of LAMP using in vitro RNA transcripts was 3.6x10 copies mu L-1, which is as sensitive as the presently used PCR assays. However, the LAMP products could be identified as different colors with the naked eye following staining with hydroxynaphthol blue dye (HNB). No cross-reactivity with other gastroenteric viruses (rotavirus and norovirus) was observed, indicating the relatively high specificity of LAMP. The RT-LAMP method with HNB was used to effectively detect astrovirus in reclaimed water samples. Conclusions: The LAMP technique described in this study is a cheap, sensitive, specific and rapid method for the detection of astrovirus. The RT-LAMP method can be simply applied for the specific detection of astrovirus and has the potential to be utilized in the field as a screening test. |
| WOS记录号 | WOS:000335265700001 |
| 公开日期 | 2015-03-26 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9591]  |
| 专题 | 生态环境研究中心_环境水质学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Yang, Bo-Yun,Liu, Xiao-Lu,Wei, Yu-Mei,et al. Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye[J]. BMC MICROBIOLOGY,2014,14(1):-. |
| APA | Yang, Bo-Yun.,Liu, Xiao-Lu.,Wei, Yu-Mei.,Wang, Jing-Qi.,He, Xiao-Qing.,...&Wang, Zi-Jian.(2014).Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye.BMC MICROBIOLOGY,14(1),-. |
| MLA | Yang, Bo-Yun,et al."Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye".BMC MICROBIOLOGY 14.1(2014):-. |
入库方式： OAI收割
来源：生态环境研究中心
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