Using high-throughput sequencing to assess the impacts of treated and untreated wastewater discharge on prokaryotic communities in an urban river
文献类型:期刊论文
作者 | Qi, Weixiao![]() ![]() |
刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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出版日期 | 2014 |
卷号 | 98期号:4页码:1841-1851 |
关键词 | Effluent-dominated river Wastewater discharge Prokaryotic community High-throughput sequencing Community composition and function |
ISSN号 | 0175-7598 |
中文摘要 | In many megacities wastewater is an important source of surface water, particularly during drought periods. While changes in surface water chemistry associated with effluent inflow have generally been well-studied, few data have been collected on the effects to prokaryotic communities. The objective of this study was to explore the impacts of treated and untreated wastewater discharges on prokaryotic community in an urban river. High-throughput sequencing was conducted for analyzing the prokaryotic community composition and function in river water, treated wastewater and untreated wastewater. Results revealed that the prokaryotic community compositions in the upstream river reach were dominated by treated wastewater discharge. In the middle-and downstream river reaches, untreated effluent volumes are higher, thus affecting the structure of the prokaryotic community, promoting a rise in Cyanobacteria and Thaumarchaeota. Function annotation revealed a number of genes associated with xenobiotic metabolism and human diseases were observed in river and wastewater samples, suggesting wastewater discharge to river may pose a risk to human health. Quantitative real-time PCR results revealed that the treated and untreated wastewater discharges also affected the abundance of ammonia oxidation bacteria (AOB) and ammonia oxidation archaea (AOA) in river. |
WOS记录号 | WOS:000332106000034 |
公开日期 | 2015-03-26 |
源URL | [http://ir.rcees.ac.cn/handle/311016/9615] ![]() |
专题 | 生态环境研究中心_环境水质学国家重点实验室 |
推荐引用方式 GB/T 7714 | Qi, Weixiao,Liang, Jinsong. Using high-throughput sequencing to assess the impacts of treated and untreated wastewater discharge on prokaryotic communities in an urban river[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2014,98(4):1841-1851. |
APA | Qi, Weixiao,&Liang, Jinsong.(2014).Using high-throughput sequencing to assess the impacts of treated and untreated wastewater discharge on prokaryotic communities in an urban river.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,98(4),1841-1851. |
MLA | Qi, Weixiao,et al."Using high-throughput sequencing to assess the impacts of treated and untreated wastewater discharge on prokaryotic communities in an urban river".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 98.4(2014):1841-1851. |
入库方式: OAI收割
来源:生态环境研究中心
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