Biosensor for Organoarsenical Herbicides and Growth Promoters
文献类型：期刊论文
| 作者 | Chen, Jian ; Sun, Samio ; Li, Chen-Zhong ; Zhu, Yong-Guan ; Rosen, Barry P. |
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 48期号:2页码:1141-1147 |
| ISSN号 | 0013-936X |
| 中文摘要 | The toxic metalloid arsenic is widely distributed in food, water, and soil. While inorganic arsenic enters the environment primarily from geochemical sources, methylarsenicals either result from microbial biotransformation of inorganic arsenic or are introduced anthropogenically. Methylarsenicals such as monosodium methylarsonic acid (MSMA) have been extensively utilized as herbicides, and aromatic arsenicals such as roxarsone (Rox) are used as growth promoters for poultry and swine. Organoarsenicals are degraded to inorganic arsenic. The toxicological effects of arsenicals depend on their oxidation state, chemical composition, and bioavailability. Here we report that the active forms are the trivalent arsenic-containing species. We constructed a whole-cell biosensor utilizing a modified ArsR repressor that is highly selective toward trivalent methyl and aromatic arsenicals., with essentially no response to inorganic arsenic. The biosensor was adapted for in vitro detection of organoarsenicals using fluorescence anisotropy of ArsR-DNA interactions. It detects bacterial biomethylation of inorganic arsenite both in vivo and in vitro with detection limits of 10(-7) M and linearity to 10(-6) M for phenylarsenite and 5 x 10(-6). M for methylarsenite. The biosensor detects reduced forms of MSMA and roxarsone and offers a practical, low cost method for detecting activate forms and breakdown products of organoarsenical herbicides and growth promoters. |
| WOS记录号 | WOS:000330205000038 |
| 公开日期 | 2015-03-26 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9631]  |
| 专题 | 生态环境研究中心_土壤环境科学实验室 |
| 推荐引用方式 GB/T 7714 | Chen, Jian,Sun, Samio,Li, Chen-Zhong,et al. Biosensor for Organoarsenical Herbicides and Growth Promoters[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2014,48(2):1141-1147. |
| APA | Chen, Jian,Sun, Samio,Li, Chen-Zhong,Zhu, Yong-Guan,&Rosen, Barry P..(2014).Biosensor for Organoarsenical Herbicides and Growth Promoters.ENVIRONMENTAL SCIENCE & TECHNOLOGY,48(2),1141-1147. |
| MLA | Chen, Jian,et al."Biosensor for Organoarsenical Herbicides and Growth Promoters".ENVIRONMENTAL SCIENCE & TECHNOLOGY 48.2(2014):1141-1147. |
入库方式： OAI收割
来源：生态环境研究中心
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