Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis
文献类型：期刊论文
| 作者 | Ye, Jun ; Chang, Yue ; Yan, Yu ; Xiong, Jie ; Xue, Xi-Mei ; Yuan, Dongxia ; Sun, Guo-Xin ; Zhu, Yong-Guan ; Miao, Wei |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 149期号:1页码:50-57 |
| 关键词 | Tetrahymena pyriformis Protozoan Arsenic Methyltransferase Methylarsine Heavy metal |
| ISSN号 | 0166-445X |
| 中文摘要 | Arsenic (As) methylation in aquatic microbes plays a major role in the biogeochemistry of As. Protozoa, especially the free-living freshwater species, are important players in aquatic ecological health. In this study, an arsenite (As(III)) methyltransferase, TpyArsM, was identified and characterized in a free-living protozoan, Tetrahymena pyriformis. In order to confirm its function, TpyarsM gene was knocked-out in Tetrahymena and was also heterologously expressed in hypersensitive E. coli; these events resulted in expected decreases in As tolerance and methylation ability, respectively. In-vitro tests revealed that purified TpyArsM protein methylated inorganic As to mono- and di- methylarsenate, and also had the novel property of producing trimethylarsenite (TMA(III)) and dimethylarsine (Me2AsH) gases. This new methyltransferase gene, identified in a species near the base of the food web, has enriched our knowledge of As methyltransferases and has great potential for bioremediation of As-contaminated environments. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS记录号 | WOS:000334989300007 |
| 公开日期 | 2015-03-26 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9639]  |
| 专题 | 生态环境研究中心_土壤环境科学实验室 |
| 推荐引用方式 GB/T 7714 | Ye, Jun,Chang, Yue,Yan, Yu,et al. Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis[J]. AQUATIC TOXICOLOGY,2014,149(1):50-57. |
| APA | Ye, Jun.,Chang, Yue.,Yan, Yu.,Xiong, Jie.,Xue, Xi-Mei.,...&Miao, Wei.(2014).Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis.AQUATIC TOXICOLOGY,149(1),50-57. |
| MLA | Ye, Jun,et al."Identification and characterization of the arsenite methyltransferase from a protozoan, Tetrahyrnena pyriformis".AQUATIC TOXICOLOGY 149.1(2014):50-57. |
入库方式： OAI收割
来源：生态环境研究中心
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