Construction of a dual fluorescence whole-cell biosensor to detect N-acyl homoserine lactones
文献类型：期刊论文
| 作者 | Deng, Xuemei ; Zhuang, Guoqiang ; Ma, Anzhou ; Yu, Qing ; Zhuang, Xuliang |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 出版日期 | 2014 |
| 卷号 | 26期号:2页码:415-422 |
| 关键词 | whole-cell biosensor quorum sensing dual fluorescence gfP mcherry indicator |
| ISSN号 | 1001-0742 |
| 中文摘要 | Detection of N-acyl homoserine lactones (AHLs) is useful for understanding quorum sensing (QS) behaviors, including biofilm formation, virulence and metabolism. For detecting AHLs and indicating the host cells in situ, we constructed the plasmid pUCGMA2T(1-4) to make a dual fluorescent whole-cell biosensor based on the AhlI/R AHL system of Pseudomonas syringae pv. syringae B728a. The plasmid contains three components: constitutively expressed P-nptII:gfp for indicating host cells, P-ahlI::mcherry that produces red fluorescence in response to AHL, and the ahlR gene that encodes an AHL regulatory protein. Meanwhile, two copies of T1-4 (four tandem copies of. a transcriptional terminator) were added into the plasmid to reduce background: The results showed that when the plasmid was placed into Escherichia coli, the dual fluorescence, whole-cell biosensor was able to respond with red fluorescence within 6 hr to 5 x 10(-8)-1 x 10(-5) mol/L of 3OC6-HSL. Bright green fluorescence indicated the host cells. Furthermore, when the plasmid was transferred to wildtype Pseudomonas PhTA125 (an AHL-producing bacterium), it also showed both green and red fluorescence. This result demonstrates that this plasmid can be used to construct whole-cell indicators that can indicate the AHL response and spatial behaviors of microbes in a microenvironmental niche. |
| WOS记录号 | WOS:000331781900022 |
| 公开日期 | 2015-03-26 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9651]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Deng, Xuemei,Zhuang, Guoqiang,Ma, Anzhou,et al. Construction of a dual fluorescence whole-cell biosensor to detect N-acyl homoserine lactones[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2014,26(2):415-422. |
| APA | Deng, Xuemei,Zhuang, Guoqiang,Ma, Anzhou,Yu, Qing,&Zhuang, Xuliang.(2014).Construction of a dual fluorescence whole-cell biosensor to detect N-acyl homoserine lactones.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,26(2),415-422. |
| MLA | Deng, Xuemei,et al."Construction of a dual fluorescence whole-cell biosensor to detect N-acyl homoserine lactones".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 26.2(2014):415-422. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。