The impacts of different long-term fertilization regimes on the bioavailability of arsenic in soil: integrating chemical approach with Escherichia coli arsRp::luc-based biosensor
文献类型：期刊论文
| 作者 | Hou, Qi-Hui ; Ma, An-Zhou ; Lv, Di ; Bai, Zhi-Hui ; Zhuang, Xu-Liang ; Zhuang, Guo-Qiang |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2014 |
| 卷号 | 98期号:13页码:6137-6146 |
| 关键词 | Long-term fertilization Arsenic (As) pollution Bioavailability Sequential extraction Microbial biosensor |
| ISSN号 | 0175-7598 |
| 中文摘要 | An Escherichia coli arsRp::luc-based biosensor was constructed to measure the bioavailability of arsenic (As) in soil. In previous induction experiments, it produced a linear response (R (2) = 0.96, P < 0.01) to As from 0.05 to 5 mu mol/L after a 2-h incubation. Then, both chemical sequential extraction, Community Bureau of Reference recommended sequential extraction procedures (BCR-SEPs) and E. coli biosensor, were employed to assess the impact of different long-term fertilization regimes containing N, NP, NPK, M (manure), and NPK + M treatments on the bioavailability of arsenic (As) in soil. Per the BCR-SEPs analysis, the application of M and M + NPK led to a significant (P < 0.01) increase of exchangeable As (2-7 times and 2-5 times, respectively) and reducible As (1.5-2.5 times and 1.5-2.3 times, respectively) compared with the no fertilization treated soil (CK). In addition, direct contact assay of E. coli biosensor with soil particles also supported that bioavailable As in manure-fertilized (M and M + NPK) soil was significantly higher (P < 0.01) than that in CK soil (7 and 9 times, respectively). Organic carbon may be the major factor governing the increase of bioavailable As. More significantly, E. coli biosensor-determined As was only 18.46-85.17 % of exchangeable As and 20.68-90.1 % of reducible As based on BCR-SEPs. In conclusion, NKP fertilization was recommended as a more suitable regime in As-polluted soil especially with high As concentration, and this E. coli arsRp::luc-based biosensor was a more realistic approach in assessing the bioavailability of As in soil since it would not overrate the risk of As to the environment. |
| WOS记录号 | WOS:000337747900030 |
| 公开日期 | 2015-03-26 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/9677]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Hou, Qi-Hui,Ma, An-Zhou,Lv, Di,et al. The impacts of different long-term fertilization regimes on the bioavailability of arsenic in soil: integrating chemical approach with Escherichia coli arsRp::luc-based biosensor[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2014,98(13):6137-6146. |
| APA | Hou, Qi-Hui,Ma, An-Zhou,Lv, Di,Bai, Zhi-Hui,Zhuang, Xu-Liang,&Zhuang, Guo-Qiang.(2014).The impacts of different long-term fertilization regimes on the bioavailability of arsenic in soil: integrating chemical approach with Escherichia coli arsRp::luc-based biosensor.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,98(13),6137-6146. |
| MLA | Hou, Qi-Hui,et al."The impacts of different long-term fertilization regimes on the bioavailability of arsenic in soil: integrating chemical approach with Escherichia coli arsRp::luc-based biosensor".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 98.13(2014):6137-6146. |
入库方式： OAI收割
来源：生态环境研究中心
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