DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA
文献类型：期刊论文
| 作者 | Su, S ; Fan, JW ; Xue, B ; Yuwen, LH ; Liu, XF ; Pan, D ; Fan, CH ; Wang, LH |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2014 |
| 卷号 | 6期号:2页码:1152—1157 |
| 关键词 | GRAPHENE NANOCRYSTALS PROTEIN NANOPARTICLES HYBRIDIZATION PROBES ASSAY |
| ISSN号 | 1944-8244 |
| 通讯作者 | iamlhyuwen@njupt.edu.cn ; iamlhwang@njupt.edu.cn |
| 英文摘要 | Herein, we report a convenient approach to developing quantum dots (QDs)-based nanosensors for DNA and micro-RNA (miRNA) detection. The DNA-QDs conjugate was prepared by a ligand-exchange method. Thiollabeled ssDNA is directly attached to the QD surface, leading to highly water-dispersible nanoconjugates. The DNA-QDs conjugate has the advantages of the excellent optical properties of QDs and well-Controlled recognition properties of DNA and can be used as a nanoprobe to construct a nanosensor for nucleic acid detection. With the addition of a target nucleic acid sequence, the fluorescence intensity of QDs was quenched by an organic quencher (BHQ(2)) via Forster resonance energy transfer. This nanosensor can detect as low as 1 fM DNA and 10 fM miRNA. Moreover, the QDs-based nanosensor exhibited excellent selectivity. It not only can effectively distinguish single-base-mismatched and random nucleic sequences but also can recognize pre-miRNA and mature miRNA. Therefore, the nanosensor has high application potential for disease diagnosis and biological analysis. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000330201900056 |
| 公开日期 | 2015-03-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/14088]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Su, S,Fan, JW,Xue, B,et al. DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(2):1152—1157. |
| APA | Su, S.,Fan, JW.,Xue, B.,Yuwen, LH.,Liu, XF.,...&Wang, LH.(2014).DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA.ACS APPLIED MATERIALS & INTERFACES,6(2),1152—1157. |
| MLA | Su, S,et al."DNA-Conjugated Quantum Dot Nanoprobe for High-Sensitivity Fluorescent Detection of DNA and micro-RNA".ACS APPLIED MATERIALS & INTERFACES 6.2(2014):1152—1157. |
入库方式： OAI收割
来源：上海应用物理研究所
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