Expression, crystallization and preliminary crystallographic data analysis of PigI, a putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia
文献类型：期刊论文
| 作者 | Han, N ; Ran, TT ; Lou, XD ; Gao, YY ; He, JH ; Tang, L ; Xu, DQ ; Wang, WW |
| 刊名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
 |
| 出版日期 | 2014 |
| 卷号 | 70页码:624—627 |
| 关键词 | CRYSTAL-STRUCTURE PROTEIN CRYSTALLIZATION BACILLUS-CEREUS BIOSYNTHESIS MARCESCENS DLTA CELL STREPTOMYCES METHYLATION APOPTOSIS |
| ISSN号 | 1744-3091 |
| 通讯作者 | dqxu@njau.edu.cn ; wwwang@njau.edu.cn |
| 英文摘要 | Prodigiosin, a member of the prodiginines, is a tripyrrole red pigment synthesized by Serratia and some other microbes. A bifurcated biosynthesis pathway of prodigiosin has been proposed in Serratia in which MBC (4-methoxy- 2,2'-bipyrrole-5-carbaldehyde) and MAP (2-methyl-3-N-amyl-pyrrole) are synthesized separately and then condensed by PigC to form prodigiosin. The first step for the synthesis of MBC is the activation of l-proline by PigI, but its catalytic mechanism has remained elusive. To elucidate its mechanism, recombinant PigI was purified and crystallized. Crystals obtained by the sitting-drop method belonged to space group P1 and diffracted to 2.0 angstrom resolution, with unit-cell parameters a = 51.2, b = 62.8, c = 91.3 angstrom, alpha = 105.1, beta = 90.1, gamma = 92.2 degrees. Matthews coefficient analysis suggested two molecules in the asymmetric unit, with a V-M of 2.6 angstrom(3) Da(-1) and a solvent content of 52.69%. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000335921500017 |
| 公开日期 | 2015-03-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/14135]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Han, N,Ran, TT,Lou, XD,et al. Expression, crystallization and preliminary crystallographic data analysis of PigI, a putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2014,70:624—627. |
| APA | Han, N.,Ran, TT.,Lou, XD.,Gao, YY.,He, JH.,...&Wang, WW.(2014).Expression, crystallization and preliminary crystallographic data analysis of PigI, a putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,70,624—627. |
| MLA | Han, N,et al."Expression, crystallization and preliminary crystallographic data analysis of PigI, a putative L-prolyl-AMP ligase from the prodigiosin synthetic pathway in Serratia".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 70(2014):624—627. |
入库方式： OAI收割
来源：上海应用物理研究所
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