How water molecules affect the catalytic activity of hydrolases - A XANES study of the local structures of peptide deformylase
文献类型：期刊论文
| 作者 | Cui, PX ; Wang, Y ; Chu, WS ; Guo, XY ; Yang, FF ; Yu, MJ ; Zhao, HF ; Dong, YH ; Xie, YN ; Gong, WM ; Wu, ZY |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2014 |
| 卷号 | 4 |
| 关键词 | X-RAY-ABSORPTION BACTERIUM LEPTOSPIRA-INTERROGANS IRON-MOLYBDENUM COFACTOR ESCHERICHIA-COLI K EDGES SPECTRA PROTEIN METAL SPECTROSCOPY INSIGHT |
| ISSN号 | 2045-2322 |
| 通讯作者 | chuws@ustc.edu.cn ; wgong@sun5.ibp.ac.cn ; wuzy@ustc.edu.cn |
| 英文摘要 | Peptide deformylase (PDF) is a prokaryotic enzyme that catalyzes the deformylation of nascent peptides generated during protein synthesis and water molecules play a key role in these hydrolases. Using X-ray absorption near edge spectroscopy (XANES) and ab initio calculations we accurately probe the local atomic environment of the metal ion binding in the active site of PDF at different pHvalues and with different metal ions. This new approach is an effective way to monitor existing correlations among functions and structural changes. We show for the first time that the enzymatic activity depends on pH values and metal ions via the bond length of the nearest coordinating water (Wat1) to the metal ion. Combining experimental and theoretical data we may claim that PDF exhibits an enhanced enzymatic activity only when the distance of the Wat1 molecule with the metal ion falls in the limited range from 2.15 to 2.55 angstrom. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000346300500006 |
| 公开日期 | 2015-03-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/14177]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Cui, PX,Wang, Y,Chu, WS,et al. How water molecules affect the catalytic activity of hydrolases - A XANES study of the local structures of peptide deformylase[J]. SCIENTIFIC REPORTS,2014,4. |
| APA | Cui, PX.,Wang, Y.,Chu, WS.,Guo, XY.,Yang, FF.,...&Wu, ZY.(2014).How water molecules affect the catalytic activity of hydrolases - A XANES study of the local structures of peptide deformylase.SCIENTIFIC REPORTS,4. |
| MLA | Cui, PX,et al."How water molecules affect the catalytic activity of hydrolases - A XANES study of the local structures of peptide deformylase".SCIENTIFIC REPORTS 4(2014). |
入库方式： OAI收割
来源：上海应用物理研究所
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