Multivalent Capture and Detection of Cancer Cells with DNA Nanostructured Biosensors and Multibranched Hybridization Chain Reaction Amplification
文献类型：期刊论文
| 作者 | Zhou, GB ; Lin, MH ; Song, P ; Chen, XQ ; Chao, J ; Wang, LH ; Huang, Q ; Huang, W ; Fan, CH ; Zuo, XL |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2014 |
| 卷号 | 86期号:15页码:7843—7848 |
| 关键词 | CIRCULATING TUMOR-CELLS QUANTUM DOTS LABEL-FREE NANOPARTICLES EXPRESSION TRANSPORT MICRORNA DENSITY PROBES ASSAY |
| ISSN号 | 0003-2700 |
| 通讯作者 | xqchen@csu.edu.cn ; zuoxiaolei@sinap.ac.cn |
| 英文摘要 | Sensitive detection of cancer cells plays a critically important role in the early detection of cancer and cancer metastasis. However, because circulating tumor cells are extremely rare in peripheral blood, the detection of cancer cells with high analytical sensitivity and specificity remains challenging. Here, we have demonstrated a simple, sensitive and specific detection of cancer cells with the detection sensitivity of four cancer cells, which is lower than the cutoff value with respect to correlation with survival outcomes as well as predictive of metastatic disease in clinical diagnostics. We re-engineered the hybridization chain reaction (HCR) to multibranched HCR (mHCR) that can produce long products with multiple biotins for signal amplification and multiple branched arms for multivalent binding. The capturing gold surface is modified with DNA tetrahedral probes, which provide superior hybridization conditions for the multivalent binding. The synergetic effect of mHCR amplification and multivalent binding lead to the high sensitivity of our detection platform. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000340081100096 |
| 公开日期 | 2015-03-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/14260]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Zhou, GB,Lin, MH,Song, P,et al. Multivalent Capture and Detection of Cancer Cells with DNA Nanostructured Biosensors and Multibranched Hybridization Chain Reaction Amplification[J]. ANALYTICAL CHEMISTRY,2014,86(15):7843—7848. |
| APA | Zhou, GB.,Lin, MH.,Song, P.,Chen, XQ.,Chao, J.,...&Zuo, XL.(2014).Multivalent Capture and Detection of Cancer Cells with DNA Nanostructured Biosensors and Multibranched Hybridization Chain Reaction Amplification.ANALYTICAL CHEMISTRY,86(15),7843—7848. |
| MLA | Zhou, GB,et al."Multivalent Capture and Detection of Cancer Cells with DNA Nanostructured Biosensors and Multibranched Hybridization Chain Reaction Amplification".ANALYTICAL CHEMISTRY 86.15(2014):7843—7848. |
入库方式： OAI收割
来源：上海应用物理研究所
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