Novel Rolling Circle Amplification and DNA Origami-Based DNA Belt-Involved Signal Amplification Assay for Highly Sensitive Detection of Prostate-Specific Antigen (PSA)
文献类型：期刊论文
| 作者 | Yan, J ; Hu, CY ; Wang, P ; Liu, R ; Zuo, XL ; Liu, XW ; Song, SP ; Fan, CH ; He, DN ; Sun, G |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2014 |
| 卷号 | 6期号:22页码:20372—20377 |
| 关键词 | NANOSCALE SHAPES CANCER MARKERS FOLDING DNA NANOTECHNOLOGY PROTEINS NANOSTRUCTURES DELIVERY PLATFORM |
| ISSN号 | 1944-8244 |
| 通讯作者 | hdnbill@sh163.net ; cjr.sungang@vip.163.com |
| 英文摘要 | Prostate-specific antigen (PSA) is one of the most important biomarkers for the early diagnosis and prognosis of prostate cancer. Although many efforts have been made to achieve significant progress for the detection of PSA, challenges including relative low sensitivity, complicated operation, sophisticated instruments, and high cost remain unsolved. Here, we have developed a strategy combining rolling circle amplification (RCA)-based DNA belts and magnetic bead-based enzyme-linked immunosorbent assay (ELISA) for the highly sensitive and specific detection of PSA. At first, a 96-base circular DNA template was designed and prepared for the following RCA. Single stranded DNA (ssDNA) products from RCA were used as scaffold strand for DNA origami, which was hybridized with three staple strands of DNA. The resulting DNA belts were conjugated with multiple enzymes for signal amplification and then employed to magnetic bead based ELISA for PSA detection. Through our strategy, as low as 50 aM of PSA can be detected with excellent specificity. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000345721400111 |
| 公开日期 | 2015-03-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/14280]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Yan, J,Hu, CY,Wang, P,et al. Novel Rolling Circle Amplification and DNA Origami-Based DNA Belt-Involved Signal Amplification Assay for Highly Sensitive Detection of Prostate-Specific Antigen (PSA)[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(22):20372—20377. |
| APA | Yan, J.,Hu, CY.,Wang, P.,Liu, R.,Zuo, XL.,...&Sun, G.(2014).Novel Rolling Circle Amplification and DNA Origami-Based DNA Belt-Involved Signal Amplification Assay for Highly Sensitive Detection of Prostate-Specific Antigen (PSA).ACS APPLIED MATERIALS & INTERFACES,6(22),20372—20377. |
| MLA | Yan, J,et al."Novel Rolling Circle Amplification and DNA Origami-Based DNA Belt-Involved Signal Amplification Assay for Highly Sensitive Detection of Prostate-Specific Antigen (PSA)".ACS APPLIED MATERIALS & INTERFACES 6.22(2014):20372—20377. |
入库方式： OAI收割
来源：上海应用物理研究所
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