Ultrasensitive Electrochemical Detection of Prostate-Specific Antigen by Using Antibodies Anchored on a DNA Nanostructural Scaffold
文献类型：期刊论文
| 作者 | Chen, XQ ; Zhou, GB ; Song, P ; Wang, JJ ; Gao, JM ; Lu, JX ; Fan, CH ; Zuo, XL |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2014 |
| 卷号 | 86期号:15页码:7337—7342 |
| 关键词 | PROMISING PLASMA BIOMARKERS ASSAY REVEALS PROSTASOMES ELECTRON-TRANSFER BINDING IMMOBILIZATION SURFACES CONSTRUCTION PROTEINS SENSORS CANCER |
| ISSN号 | 0003-2700 |
| 通讯作者 | zuoxiaolei@sinap.ac.cn |
| 英文摘要 | The high occurrence of prostate cancer in men makes the prostate-specific antigen (PSA) screening test really important. More importantly, the recurrence rate after radical prostatectomy is high, whereas the traditional P SA immunoassay does not possess the sufficient high sensitivity for post-treatment PSA detection. In these assays, uncontrolled and random orientation of capture antibodies on the surface largely reduces their activity. Here, by exploiting the rapidly emerging DNA nanotechnology, we developed a DNA nanostructure based scaffold to precisely control the assembly of antibody monolayer. We demonstrated that the detection sensitivity was critically dependent on the nanoscale-spacing (nanospacing) of immobilized antibodies. In addition to the controlled assembly, we further amplified the sensing signal by using the gold nanoparticles, resulting in extremely high sensitivity and a low detection limit of 1 pg/mL. To test the real-world applicability of our nanoengineered electrochemical sensor, we evaluated the performance with 11 patients' serum samples and obtained consistent results with the "gold-standard" assays. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000340081100030 |
| 公开日期 | 2015-03-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/14536]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Chen, XQ,Zhou, GB,Song, P,et al. Ultrasensitive Electrochemical Detection of Prostate-Specific Antigen by Using Antibodies Anchored on a DNA Nanostructural Scaffold[J]. ANALYTICAL CHEMISTRY,2014,86(15):7337—7342. |
| APA | Chen, XQ.,Zhou, GB.,Song, P.,Wang, JJ.,Gao, JM.,...&Zuo, XL.(2014).Ultrasensitive Electrochemical Detection of Prostate-Specific Antigen by Using Antibodies Anchored on a DNA Nanostructural Scaffold.ANALYTICAL CHEMISTRY,86(15),7337—7342. |
| MLA | Chen, XQ,et al."Ultrasensitive Electrochemical Detection of Prostate-Specific Antigen by Using Antibodies Anchored on a DNA Nanostructural Scaffold".ANALYTICAL CHEMISTRY 86.15(2014):7337—7342. |
入库方式： OAI收割
来源：上海应用物理研究所
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