Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality
文献类型：期刊论文
| 作者 | Guo, YZ ; Sun, LH ; Oberthuer, D ; Zhang, CY ; Shi, JY ; Di, JL ; Zhang, BL ; Cao, HL ; Liu, YM ; Li, J ; Wang, Q ; Huang, HH ; Liu, J ; Schulz, JM ; Zhang, QY ; Zhao, JL ; Betzel, C ; He, JH ; Yin, DC |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2014 |
| 卷号 | 4 |
| 关键词 | DYNAMIC LIGHT-SCATTERING HETEROGENEOUS NUCLEATION SEPARATING NUCLEATION STRUCTURAL GENOMICS LYSOZYME SURFACES OPTIMIZATION ORIENTATION NUCLEANTS GROWTH |
| ISSN号 | 2045-2322 |
| 通讯作者 | Christian.Betzel@uni-hamburg.de ; hejianhua@sinap.ac.cn ; yindc@nwpu.edu.cn |
| 英文摘要 | High-quality protein crystals of suitable size are an important prerequisite for applying X-ray crystallography to determine the 3-dimensional structure of proteins. However, it is often difficult to obtain protein crystals of appropriate size and quality because nucleation and growth processes can be unsuccessful. Here, we show that by adsorbing proteins onto porous polystyrene-divinylbenzene microspheres (SDB) floating on the surface of the crystallisation solution, a localised high supersaturation region at the surface of the microspheres and a low supersaturation region below the microspheres can coexist in a single solution. The crystals will easily nucleate in the region of high supersaturation, but when they grow to a certain size, they will sediment to the region of low supersaturation and continue to grow. In this way, the probability of crystallisation and crystal quality can be simultaneously increased in a single solution without changing other crystallisation parameters. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000346267700002 |
| 公开日期 | 2015-03-13 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/14548]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Guo, YZ,Sun, LH,Oberthuer, D,et al. Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality[J]. SCIENTIFIC REPORTS,2014,4. |
| APA | Guo, YZ.,Sun, LH.,Oberthuer, D.,Zhang, CY.,Shi, JY.,...&Yin, DC.(2014).Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality.SCIENTIFIC REPORTS,4. |
| MLA | Guo, YZ,et al."Utilisation of adsorption and desorption for simultaneously improving protein crystallisation success rate and crystal quality".SCIENTIFIC REPORTS 4(2014). |
入库方式： OAI收割
来源：上海应用物理研究所
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