Label-free, real-time detection of the dynamic processes of protein degradation using oblique-incidence reflectivity difference method
文献类型：期刊论文
| 作者 | Liu, S ; Zhu, JH ; He, LP ; Dai, J ; Lu, HB ; Wu, L ; Jin, KJ ; Yang, GZ ; Zhu, H |
| 刊名 | APPLIED PHYSICS LETTERS
 |
| 出版日期 | 2014 |
| 卷号 | 104期号:16 |
| ISSN号 | 0003-6951 |
| 通讯作者 | Lu, HB (reprint author), Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Inst Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | Based on the requirements for studying the dynamic process of proteinase action substrates in life science, we selected six random proteins including 1L-10, SCGB2A2, CENPQ, GST, HK1, KLHL7, as well as five different concentrations of 1L-10 proteins of 1 mg/ml, 0.5 mg/ml, 0.25 mg/ml, 0.125 mg/ml, and 0.0625 mg/ml, and fabricated two types of substrate protein microarrays, respectively. We detected the dynamic processes of proteins degraded by proteinase K using oblique-incidence reflectivity difference (OIRD) method in a label-free and real-time manner. We obtained the relevant degradation velocities and the degradation time. The experimental results demonstrate that OIRD has the ability to study proteinase action substrates which is out of reach of label methods and is expected to offer opportunities to determine protease-substrate relationships on the systems biology level. (C) 2014 AIP Publishing LLC. |
| 资助信息 | National Basic Research Program of China [2007CB935701]; NIH [U54 HG006434] |
| 语种 | 英语 |
| 公开日期 | 2015-04-14 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/58916]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu, S,Zhu, JH,He, LP,et al. Label-free, real-time detection of the dynamic processes of protein degradation using oblique-incidence reflectivity difference method[J]. APPLIED PHYSICS LETTERS,2014,104(16). |
| APA | Liu, S.,Zhu, JH.,He, LP.,Dai, J.,Lu, HB.,...&Zhu, H.(2014).Label-free, real-time detection of the dynamic processes of protein degradation using oblique-incidence reflectivity difference method.APPLIED PHYSICS LETTERS,104(16). |
| MLA | Liu, S,et al."Label-free, real-time detection of the dynamic processes of protein degradation using oblique-incidence reflectivity difference method".APPLIED PHYSICS LETTERS 104.16(2014). |
入库方式： OAI收割
来源：物理研究所
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