Interaction Study between DNA and Histone Proteins on Single-molecule Level using Atomic Force Microscopy
文献类型：期刊论文
| 作者 | Liu, YY ; Wang, PY ; Dou, SX ; Lv, HF |
| 刊名 | CHINESE JOURNAL OF CHEMICAL PHYSICS
 |
| 出版日期 | 2014 |
| 卷号 | 27期号:1页码:115 |
| 关键词 | DNA Histone Atomic force microscopy Single molecule Dialysis |
| ISSN号 | 1674-0068 |
| 通讯作者 | Liu, YY (reprint author), China Agr Univ, Coll Sci, Beijing 100083, Peoples R China. |
| 中文摘要 | DNA and histone protein are important in the formation of nucleosomal arrays, which are the first packaging level of DNA into a more compact chromatin structure. To characterize the interactions of DNA and histone proteins, we reconstitute nucleosomes using lambda DNA and whole histone proteins by dialysis and perform direct atomic force microscopy (AFM) imaging. Compared with non-specific DNA and histone binding, nucleosomes are formed within the assembled "beads-on-a-string" nucleosomal array by dialysis. These observations facilitate the establishment of the molecular mechanisms of nucleosome and demonstrate the capability of AFM for protein-DNA interaction analysis. |
| 资助信息 | National Natural Science Foundation of China [11274374]; National Basic Research Program of China [2009CB930704]; Basic Scientific Research Foundation of China Agricultural University [2012QJ026] |
| 语种 | 英语 |
| 公开日期 | 2015-04-14 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/58963]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Liu, YY,Wang, PY,Dou, SX,et al. Interaction Study between DNA and Histone Proteins on Single-molecule Level using Atomic Force Microscopy[J]. CHINESE JOURNAL OF CHEMICAL PHYSICS,2014,27(1):115. |
| APA | Liu, YY,Wang, PY,Dou, SX,&Lv, HF.(2014).Interaction Study between DNA and Histone Proteins on Single-molecule Level using Atomic Force Microscopy.CHINESE JOURNAL OF CHEMICAL PHYSICS,27(1),115. |
| MLA | Liu, YY,et al."Interaction Study between DNA and Histone Proteins on Single-molecule Level using Atomic Force Microscopy".CHINESE JOURNAL OF CHEMICAL PHYSICS 27.1(2014):115. |
入库方式： OAI收割
来源：物理研究所
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