Parallel detection and quantitative analysis of specific binding of proteins by oblique-incidence reflectivity difference technique in label-free format
文献类型：期刊论文
| 作者 | Dai, J ; Li, L ; He, LP ; Ruan, KC ; Lu, HB ; Jin, KJ ; Yang, GZ |
| 刊名 | SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
 |
| 出版日期 | 2014 |
| 卷号 | 57期号:11页码:2039 |
| 关键词 | oblique-incidence reflectivity difference (OIRD) protein microarray label-free detection quantitative analysis |
| ISSN号 | 1674-7348 |
| 通讯作者 | Lu, HB (reprint author), Chinese Acad Sci, Beijing Natl Lab Condensed Matter Phys, Inst Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | In this work, we parallelly detected the specific binding between microarray targets including 12 different kinds of proteins and the probe solution containing five corresponding antibodies and quantitatively analyzed the interactions between CDH13 and solution phase anti-CDH13 at six different probe concentrations by oblique-incidence reflectivity difference (OIRD) method in label-free format. The detection sensitivity reached 10 ng/mL. The experimental results indicate that the OIRD method is a promising and competing technique not only in research work but also in clinic. |
| 资助信息 | National Basic Research Program of China [2007CB935700] |
| 语种 | 英语 |
| 公开日期 | 2015-04-14 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/59662]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Dai, J,Li, L,He, LP,et al. Parallel detection and quantitative analysis of specific binding of proteins by oblique-incidence reflectivity difference technique in label-free format[J]. SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2014,57(11):2039. |
| APA | Dai, J.,Li, L.,He, LP.,Ruan, KC.,Lu, HB.,...&Yang, GZ.(2014).Parallel detection and quantitative analysis of specific binding of proteins by oblique-incidence reflectivity difference technique in label-free format.SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,57(11),2039. |
| MLA | Dai, J,et al."Parallel detection and quantitative analysis of specific binding of proteins by oblique-incidence reflectivity difference technique in label-free format".SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY 57.11(2014):2039. |
入库方式： OAI收割
来源：物理研究所
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