Label-free high-throughput and real-time detections of protein interactions by oblique-incidence reflectivity difference method
文献类型：期刊论文
| 作者 | He, LP ; Liu, S ; Dai, J ; Lu, HB ; Jin, KJ ; Yang, GZ |
| 刊名 | SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY
 |
| 出版日期 | 2014 |
| 卷号 | 57期号:4页码:615 |
| 关键词 | oblique-incidence reflectivity difference (OIRD) label-free real-time high-throughput |
| ISSN号 | 1674-7348 |
| 通讯作者 | Lu, HB (reprint author), Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | Selected Mouse IgG of 1 mg/mL as target was fabricated on microarray for 500 sample dots. Label-free and real-time reaction dynamic processes were detected between the microarrays with Goat Anti-mouse IgG of 0.02 mg/mL using the oblique-incidence reflectivity difference (OIRD) method. We obtained the reaction results and the reaction dynamic curves of 500 protein dots. In addition, we also used label-free detection of protein microarrays of 10080 sample dots, including BSA and different concentrations of Mouse IgG and Rabbit IgG, by OIRD. The obtained reaction results between the protein microarray with 1 mg/mL Goat Anti-mouse IgG and 1 mg/mL Goat Anti-rabbit IgG are reported herein. Experimental results show that OIRD can be not only label-free high-throughput detection method for biological microarrays but also label-free real-time detection in the interaction processes of biomolecules. |
| 资助信息 | National Basic Research Program of China [2007CB35710] |
| 语种 | 英语 |
| 公开日期 | 2015-04-14 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/59672]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | He, LP,Liu, S,Dai, J,et al. Label-free high-throughput and real-time detections of protein interactions by oblique-incidence reflectivity difference method[J]. SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,2014,57(4):615. |
| APA | He, LP,Liu, S,Dai, J,Lu, HB,Jin, KJ,&Yang, GZ.(2014).Label-free high-throughput and real-time detections of protein interactions by oblique-incidence reflectivity difference method.SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY,57(4),615. |
| MLA | He, LP,et al."Label-free high-throughput and real-time detections of protein interactions by oblique-incidence reflectivity difference method".SCIENCE CHINA-PHYSICS MECHANICS & ASTRONOMY 57.4(2014):615. |
入库方式： OAI收割
来源：物理研究所
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