Thermal-triggerd Proteinquake Leads to Disassembly of DegP Hexamer as an Imperative Activation Step
文献类型：期刊论文
| 作者 | Li, SS ; Wang, R ; Li, DY ; Ma, J ; Li, H ; He, XC ; Chang, ZY ; Weng, YX |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2014 |
| 卷号 | 4 |
| ISSN号 | 2045-2322 |
| 通讯作者 | Weng, YX (reprint author), Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing 100190, Peoples R China. |
| 中文摘要 | The Escherichia coli DegP has been reported to function both as molecular chaperone and protease for the quality control of outer membrane protein biogenesis. Activation of the inactive DegP hexamers was believed to occur via their disassembly into trimeric units and subsequent reassembly into larger oligomers (12-mers and 24-mers). Here, we analyzed the thermal stability and the unfolding dynamics of the different secondary structure components of the DegP hexamers using Fourier transform infrared spectroscopy and temperature-jump nanosecond time-resolved IR difference absorbance spectroscopy. We found that the interfacial secondary structure components possess a degreed thermal stability, with the disassembly of the DegP hexamers follows a "proteinquake'' manner, such that the fully exposed parts of the interfacial beta-sheets serving as the temperature sensor and epicenter to drive the sequential unfolding/disassembly process that finishes within about 134 ns at room temperature. |
| 资助信息 | Natural Science Foundation of China [20925313, 21090342, 31170738]; National Basic Research Program of China [2006CB910302, 2012CB917300]; Chinese Academy of Sciences Innovation Program [KJCX2-YW-W25] |
| 语种 | 英语 |
| 公开日期 | 2015-04-14 |
| 源URL | [http://ir.iphy.ac.cn/handle/311004/59721]  |
| 专题 | 物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li, SS,Wang, R,Li, DY,et al. Thermal-triggerd Proteinquake Leads to Disassembly of DegP Hexamer as an Imperative Activation Step[J]. SCIENTIFIC REPORTS,2014,4. |
| APA | Li, SS.,Wang, R.,Li, DY.,Ma, J.,Li, H.,...&Weng, YX.(2014).Thermal-triggerd Proteinquake Leads to Disassembly of DegP Hexamer as an Imperative Activation Step.SCIENTIFIC REPORTS,4. |
| MLA | Li, SS,et al."Thermal-triggerd Proteinquake Leads to Disassembly of DegP Hexamer as an Imperative Activation Step".SCIENTIFIC REPORTS 4(2014). |
入库方式： OAI收割
来源：物理研究所
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