In vivo biochemistry applications for small molecule biosensors in plant biology
文献类型：期刊论文
| 作者 | Jones, A. M. |
| 刊名 | Current Opinion in Plant Biology
 |
| 出版日期 | 2013 |
| 卷号 | 16期号:3页码:389-395 |
| ISSN号 | 1369-5266 |
| 中文摘要 | Revolutionary new technologies, namely in the areas of DNA sequencing and molecular imaging, continue to impact new discoveries in plant science and beyond. For decades we have been able to determine properties of enzymes, receptors and transporters in vitro or in heterologous systems, and more recently been able to analyze their regulation at the transcriptional level, to use GFP reporters for obtaining insights into cellular and subcellular localization, and tp measure ion and metabolite levels with unprecedented precision using mass spectrometry. However, we lack key information on the location and dynamics of the substrates of enzymes, receptors and transporters, and on the regulation of these proteins in their cellular environment. Such information can now be obtained by transitioning from in vitro to in vivo biochemistry using biosensors. Genetically encoded fluorescent protein-based sensors for ion and metabolite dynamics provide highly resolved spatial and temporal information, and are complemented by sensors for pH, redox, voltage, and tension. They serve as powerful tools for identifying missing processes (e.g., glucose transport across ER membranes), components (e.g., SWEET sugar transporters for cellular sugar efflux), and signaling networks (e.g., from systematic screening of mutants that affect sugartransport or cytosolic and vacuolar pH). Combined with the knowledge of properties of enzymes and transporters and their interactions with the regulatory machinery, biosensors promise to be key diagnostic tools for systems and synthetic biology. |
| 语种 | 英语 |
| 公开日期 | 2015-04-03 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/8585]  |
| 专题 | 西双版纳热带植物园_公共技术服务中心主要仪器相关文献_植物活体成像系统 |
| 推荐引用方式 GB/T 7714 | Jones, A. M.. In vivo biochemistry applications for small molecule biosensors in plant biology[J]. Current Opinion in Plant Biology,2013,16(3):389-395. |
| APA | Jones, A. M..(2013).In vivo biochemistry applications for small molecule biosensors in plant biology.Current Opinion in Plant Biology,16(3),389-395. |
| MLA | Jones, A. M.."In vivo biochemistry applications for small molecule biosensors in plant biology".Current Opinion in Plant Biology 16.3(2013):389-395. |
入库方式： OAI收割
来源：西双版纳热带植物园
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