Quantification of Forster resonance energy transfer by monitoring sensitized emission in living plant cells
文献类型：期刊论文
| 作者 | Mueller, S. |
| 刊名 | Frontiers in Plant Science
 |
| 出版日期 | 2013 |
| 卷号 | 4页码:20 |
| ISSN号 | 1664-462X |
| 中文摘要 | Forster resonance energy transfer (FRET) describes excitation energy exchange between two adjacent molecules typically in distances ranging from 2 to 10 nm. The process depends on dipole-dipole coupling of the molecules and its probability of occurrence cannot be proven directly. Mostly, fluorescence is employed for quantification as it represents a concurring process of relaxation of the excited singlet state Si so that the probability of fluorescence decreases as the probability of FRET increases. This reflects closer proximity of the molecules or an orientation of donor and acceptor transition dipoles that facilitates FRET Monitoring sensitized emission by 3-Filter-FRET allows for fast image acquisition and is suitable for quantifying FRET in dynamic systems such as living cells. In recent years, several calibration protocols were established to overcome to previous difficulties in measuring FRET-efficiencies. Thus, we can now obtain by 3-filter FRET FRET-efficiencies that are comparable to results from sophisticated fluorescence lifetime measurements. With the discovery of fluorescent proteins and their improvement toward spectral variants and usability in plant cells, the tool box for in vivo FRET-analyses in plant cells was provided and FRET became applicable for the in vivo detection of protein-protein interactions and for monitoring conformational dynamics. The latter opened the door toward a multitude of FRET-sensors such as the widely applied Ca2+-sensor Cameleon. Recently, FRET-couples of two fluorescent proteins were supplemented by additional fluorescent proteins toward FRET-cascades in order to monitor more complex arrangements. Novel FRET-couples involving switchable fluorescent proteins promise to increase the utility of FRET through combination with photoactivation-based super-resolution microscopy. |
| 语种 | 英语 |
| 公开日期 | 2015-04-03 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/8588]  |
| 专题 | 西双版纳热带植物园_公共技术服务中心主要仪器相关文献_植物活体成像系统 |
| 推荐引用方式 GB/T 7714 | Mueller, S.. Quantification of Forster resonance energy transfer by monitoring sensitized emission in living plant cells[J]. Frontiers in Plant Science,2013,4:20. |
| APA | Mueller, S..(2013).Quantification of Forster resonance energy transfer by monitoring sensitized emission in living plant cells.Frontiers in Plant Science,4,20. |
| MLA | Mueller, S.."Quantification of Forster resonance energy transfer by monitoring sensitized emission in living plant cells".Frontiers in Plant Science 4(2013):20. |
入库方式： OAI收割
来源：西双版纳热带植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。