Fusion, rupture, and degeneration the fate of in vivo-labelled PSVs in developing barley endosperm
文献类型：期刊论文
| 作者 | Ibl, V. |
| 刊名 | Journal of Experimental Botany
 |
| 出版日期 | 2014 |
| 卷号 | 65期号:12页码:3249-3261 |
| ISSN号 | 0022-0957 |
| 中文摘要 | Cereal endosperm is a highly differentiated tissue containing specialized organelles for the accumulation of storage proteins. The endosperm of barley contains hordeins, which are ultimately deposited within protein storage vacuoles (PSVs). These organelles have been characterized predominantly by the histochemical analysis of fixed immature tissue samples. However, little is known about the fate of PSVs during barley endosperm development, and in vivo imaging has not been attempted in order to gain further insight. In this report, young seeds were followed through development to characterize the dynamic morphology of PSVs from aleurone, subaleurone, and central starchy endosperm cells. TIP3-GFP was used as a PSV membrane marker and several fluorescent tracers were used to identify membranes and monitor endomembrane organelles in real time. Whereas the spherical appearance of strongly labelled TIP3-GFP PSVs in the aleurone remained constant, those in the subaleurone and central starchy endosperm underwent substantial morphological changes. Fusion and rupture events were observed in the subaleurone, and internal membranes derived from both the tonoplast and endoplasmic reticulum were identified within these PSVs. TIP3-GFP-labelled PSVs in the starchy endosperm cells underwent a dramatic reduction in size, so that finally the protein bodies were tightly enclosed. Potential desiccation-related membrane-altering processes that may be causally linked to these dynamic endomembrane events in the barley endosperm are discussed. |
| 语种 | 英语 |
| 公开日期 | 2015-04-03 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/8591]  |
| 专题 | 西双版纳热带植物园_公共技术服务中心主要仪器相关文献_植物活体成像系统 |
| 推荐引用方式 GB/T 7714 | Ibl, V.. Fusion, rupture, and degeneration the fate of in vivo-labelled PSVs in developing barley endosperm[J]. Journal of Experimental Botany,2014,65(12):3249-3261. |
| APA | Ibl, V..(2014).Fusion, rupture, and degeneration the fate of in vivo-labelled PSVs in developing barley endosperm.Journal of Experimental Botany,65(12),3249-3261. |
| MLA | Ibl, V.."Fusion, rupture, and degeneration the fate of in vivo-labelled PSVs in developing barley endosperm".Journal of Experimental Botany 65.12(2014):3249-3261. |
入库方式： OAI收割
来源：西双版纳热带植物园
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