Robust anti-oxidant defences in the rice blast fungus Magnaporthe oryzae confer tolerance to the host oxidative burst
文献类型：期刊论文
| 作者 | Samalova, M. |
| 刊名 | New Phytologist
 |
| 出版日期 | 2014 |
| 卷号 | 201期号:2页码:556-573 |
| ISSN号 | 1469-8137 |
| 中文摘要 | Plants respond to pathogen attack via a rapid burst of reactive oxygen species (ROS). However, ROS are also produced by fungal metabolism and are required for the development of infection structures in Magnaporthe oryzae. To obtain a better understanding of redox regulation in M.oryzae, we measured the amount and redox potential of glutathione (E-GSH), as the major cytoplasmic anti-oxidant, the rates of ROS production, and mitochondrial activity using multi-channel four-dimensional (x,y,z,t) confocal imaging of Grx1-roGFP2 and fluorescent reporters during spore germination, appressorium formation and infection. High levels of mitochondrial activity and ROS were localized to the growing germ tube and appressorium, but E-GSH was highly reduced and tightly regulated during development. Furthermore, germlings were extremely resistant to external H2O2 exposure explanta. E-GSH remained highly reduced during successful infection of the susceptible rice cultivar CO39. By contrast, there was a dramatic reduction in the infection of resistant (IR68) rice, but the sparse hyphae that did form also maintained a similar reduced E-GSH. We conclude that M.oryzae has a robust anti-oxidant defence system and maintains tight control of E-GSH despite substantial oxidative challenge. Furthermore, the magnitude of the host oxidative burst alone does not stress the pathogen sufficiently to prevent infection in this pathosystem. |
| 公开日期 | 2015-04-03 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/8594]  |
| 专题 | 西双版纳热带植物园_公共技术服务中心主要仪器相关文献_植物活体成像系统 |
| 推荐引用方式 GB/T 7714 | Samalova, M.. Robust anti-oxidant defences in the rice blast fungus Magnaporthe oryzae confer tolerance to the host oxidative burst[J]. New Phytologist,2014,201(2):556-573. |
| APA | Samalova, M..(2014).Robust anti-oxidant defences in the rice blast fungus Magnaporthe oryzae confer tolerance to the host oxidative burst.New Phytologist,201(2),556-573. |
| MLA | Samalova, M.."Robust anti-oxidant defences in the rice blast fungus Magnaporthe oryzae confer tolerance to the host oxidative burst".New Phytologist 201.2(2014):556-573. |
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来源：西双版纳热带植物园
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