中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia

文献类型:期刊论文

作者Xu, SZ ; Li, DZ ; Li, JW ; Xiang, XG ; Jin, WT ; Huang, WC ; Jin, XH ; Huang, LQ
刊名PLOS ONE
出版日期2015
卷号10期号:1页码:-
关键词INTERNAL TRANSCRIBED SPACER LAND PLANTS PHYLOGENETIC ANALYSIS RIBOSOMAL DNA SEQUENCES SYSTEMATICS IDENTIFICATION DISCRIMINATE TAXONOMY RATES
ISSN号1932-6203
中文摘要DNA barcoding has been proposed to be one of the most promising tools for accurate and rapid identification of taxa. However, few publications have evaluated the efficiency of DNA barcoding for the large genera of flowering plants. Dendrobium, one of the largest genera of flowering plants, contains many species that are important in horticulture, medicine and biodiversity conservation. Besides, Dendrobium is a notoriously difficult group to identify. DNA barcoding was expected to be a supplementary means for species identification, conservation and future studies in Dendrobium. We assessed the power of 11 candidate barcodes on the basis of 1,698 accessions of 184 Dendrobium species obtained primarily from mainland Asia. Our results indicated that five single barcodes, i.e., ITS, ITS2, matK, rbcL and trnH-psbA, can be easily amplified and sequenced with the currently established primers. Four barcodes, ITS, ITS2, ITS+matK, and ITS2+matK, have distinct barcoding gaps. ITS+matK was the optimal barcode based on all evaluation methods. Furthermore, the efficiency of ITS+matK was verified in four other large genera including Ficus, Lysimachia, Paphiopedilum, and Pedicularis in this study. Therefore, we tentatively recommend the combination of ITS+ matK as a core DNA barcode for large flowering plant genera.
原文出处10.1371/journal.pone.0115168
语种英语
公开日期2015-03-26
源URL[http://ir.xtbg.org.cn/handle/353005/8381]  
专题西双版纳热带植物园_支撑系统
推荐引用方式
GB/T 7714
Xu, SZ,Li, DZ,Li, JW,et al. Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia[J]. PLOS ONE,2015,10(1):-.
APA Xu, SZ.,Li, DZ.,Li, JW.,Xiang, XG.,Jin, WT.,...&Huang, LQ.(2015).Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia.PLOS ONE,10(1),-.
MLA Xu, SZ,et al."Evaluation of the DNA Barcodes in Dendrobium (Orchidaceae) from Mainland Asia".PLOS ONE 10.1(2015):-.

入库方式: OAI收割

来源:西双版纳热带植物园

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