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KChIP-Like Auxiliary Subunits of Kv4 Channels Regulate Excitability of Muscle Cells and Control Male Turning Behavior during Mating in Caenorhabditis elegans
文献类型：期刊论文
| 作者 | Chen, Xin; Ruan, Mei-Yu; Cai, Shi-Qing |
| 刊名 | JOURNAL OF NEUROSCIENCE
 |
| 出版日期 | 2015 |
| 卷号 | 35期号:5页码:1880-1891 |
| 关键词 | POTASSIUM CHANNELS K+ CHANNEL CARDIAC-ARRHYTHMIA PRIMARY CULTURE INACTIVATION NEURONS FAMILY MODULATION DEPENDENCE COMPLEXES |
| 通讯作者 | Cai, SQ (reprint author), 320 Yueyang Rd,Biol Res Bldg,Room 505A, Shanghai 200031, Peoples R China. |
| 英文摘要 | Voltage-gated Kv4 channels control the excitability of neurons and cardiac myocytes by conducting rapidly activating-inactivating currents. The function of Kv4 channels is profoundly modulated by K+ channel interacting protein (KChIP) soluble auxiliary subunits. However, the in vivo mechanism of the modulation is not fully understood. Here, we identified three C. elegans KChIP-like (ceKChIP) proteins, NCS-4, NCS-5, and NCS-7. All three ceKChIPs alter electrical characteristics of SHL-1, a C. elegans Kv4 channel ortholog, currents by slowing down inactivation kinetics and shifting voltage dependence of activation to more hyperpolarizing potentials. Native SHL-1 current is completely abolished in cultured myocytes of Triple KO worms in which all three ceKChIP genes are deleted. Reexpression of NCS-4 partially restored expression of functional SHL-1 channels, whereas NCS-4(efm), a NCS-4 mutant with impaired Ca2+-binding ability, only enhanced expression of SHL-1 proteins, but failed to transport them from the Golgi apparatus to the cell membrane in body wall muscles of Triple KO worms. Moreover, translational reporter revealed that NCS-4 assembles with SHL-1 K+ channels in male diagonal muscles. Deletion of either ncs-4 or shl-1 significantly impairs male turning, a behavior controlled by diagonal muscles during mating. The phenotype of the ncs-4 null mutant could be rescued by reexpression of NCS-4, but not NCS-4(efm), further emphasizing the importance of Ca2+ binding to ceKChIPs in regulating native SHL-1 channel function. Together, these data reveal an evolutionarily conserved mechanism underlying the regulation of Kv4 channels by KChIPs and unravel critical roles of ceKChIPs in regulating muscle cell excitability and animal behavior in C. elegans. |
| WOS标题词 | Neurosciences & Neurology |
| 学科主题 | Neurosciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000349671100007 |
| 公开日期 | 2015-05-27 |
| 源URL | [http://ir.sibs.ac.cn/handle/331001/2663]  |
| 专题 | 上海神经科学研究所_神经所(总) |
| 推荐引用方式 GB/T 7714 | Chen, Xin,Ruan, Mei-Yu,Cai, Shi-Qing. KChIP-Like Auxiliary Subunits of Kv4 Channels Regulate Excitability of Muscle Cells and Control Male Turning Behavior during Mating in Caenorhabditis elegans[J]. JOURNAL OF NEUROSCIENCE,2015,35(5):1880-1891. |
| APA | Chen, Xin,Ruan, Mei-Yu,&Cai, Shi-Qing.(2015).KChIP-Like Auxiliary Subunits of Kv4 Channels Regulate Excitability of Muscle Cells and Control Male Turning Behavior during Mating in Caenorhabditis elegans.JOURNAL OF NEUROSCIENCE,35(5),1880-1891. |
| MLA | Chen, Xin,et al."KChIP-Like Auxiliary Subunits of Kv4 Channels Regulate Excitability of Muscle Cells and Control Male Turning Behavior during Mating in Caenorhabditis elegans".JOURNAL OF NEUROSCIENCE 35.5(2015):1880-1891. |
入库方式： OAI收割
来源：上海神经科学研究所
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