The Volatile Oil of Nardostachyos Radix et Rhizoma Induces Endothelial Nitric Oxide Synthase Activity in HUVEC Cells
文献类型：期刊论文
| 作者 | Maiwulanjiang, M (Maiwulanjiang, Maitinuer) ; Bi, CWC (Bi, Cathy W. C.); Lee, PSC (Lee, Pinky S. C.); Xin, G (Xin, Guizhong); Miernisha, A (Miernisha, Abudureyimu); Lau, KM (Lau, Kei M.); Xiong, A (Xiong, Aizhen); Li, N (Li, Ning); Dong, TTX (Dong, Tina T. X.); Aisa, HA (Aisa, Haji A.)
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| 刊名 | PLOS ONE
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| 出版日期 | 2015 |
| 卷号 | 10期号:2 |
| 关键词 | PHOSPHORYLATION INJURY AKT IDENTIFICATION ACTIVATION JATAMANSI PATHWAY SYSTEM OXYGEN ENOS |
| ISSN号 | 1932-6203 |
| 中文摘要 | Nardostahyos Radix et Rhizoma (NRR; the root and rhizome of Nardostachys jatamansi DC.) is a widely used medicinal herb. Historically, NRR is being used for the treatment of cardiovascular and neurological diseases. To search for active ingredients of NRR, we investigated the vascular benefit of NRR volatile oil in (i) the vasodilation in rat aorta ring, and (ii) the release of nitric oxide (NO) and the phosphorylation of endothelial NO synthase (eNOS) in cultured human umbilical vein endothelial cells (HUVECs). By measuring the fluorescence signal in cultures, application of NRR volatile oil resulted in a rapid activation of NO release as well as the phosphorylation of eNOS: both inductions were markedly reduced by L-NAME. In parallel, the phosphorylation level of Akt kinase was markedly increased by the oil treatment, which was partially attenuated by PI3K/Akt inhibitor LY294002. This inhibitor also blocked the NRR-induced NO production and eNOS phosphorylation. In HUVECs, application of NRR volatile oil elevated the intracellular Ca2+ level, and BAPTA-AM, a Ca2+ chelator, reduced the Ca2+ surge: the blockage were also applied to NRR-induced eNOS phosphorylation and NO production. These findings suggested the volatile oil of NRR was the major ingredient in triggering the vascular dilatation, and which was mediated via the NO production. |
| 收录类别 | SCI |
| 资助信息 | Hong Kong Research Grants Council Theme-based Research Scheme；GRF ；Hong Kong Jockey Club Charities Trust；Foundation of The Awareness of Nature |
| 公开日期 | 2015-03-10 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/4205]  |
| 专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 新疆理化技术研究所_资源化学研究室 |
| 作者单位 | Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Peoples R China;Hong Kong Univ Sci & Technol, Ctr Chinese Med, Hong Kong, Peoples R China;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Maiwulanjiang, M ,Bi, CWC ,Lee, PSC ,et al. The Volatile Oil of Nardostachyos Radix et Rhizoma Induces Endothelial Nitric Oxide Synthase Activity in HUVEC Cells[J]. PLOS ONE,2015,10(2). |
| APA | Maiwulanjiang, M .,Bi, CWC .,Lee, PSC .,Xin, G .,Miernisha, A .,...&Aisa, HA .(2015).The Volatile Oil of Nardostachyos Radix et Rhizoma Induces Endothelial Nitric Oxide Synthase Activity in HUVEC Cells.PLOS ONE,10(2). |
| MLA | Maiwulanjiang, M ,et al."The Volatile Oil of Nardostachyos Radix et Rhizoma Induces Endothelial Nitric Oxide Synthase Activity in HUVEC Cells".PLOS ONE 10.2(2015). |
入库方式： OAI收割
来源：新疆理化技术研究所
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