Dual sensitive and temporally controlled camptothecin prodrug liposomes codelivery of siRNA for high efficiency tumor therapy
文献类型：期刊论文
| 作者 | Li, Yan1,2; Liu, Ruiyuan1,3; Yang, Jun1; Ma, Guanghui1; Zhang, Zhenzhong3; Zhang, Xin1 |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 35期号:36页码:9731-9745 |
| 关键词 | Dual sensitive Camptothecin prodrug Poly(carboxybetaine) Temporally controlled release Combination therapy |
| ISSN号 | 0142-9612 |
| 其他题名 | Biomaterials |
| 中文摘要 | The combination of chemotherapeutic drug camptothecin (CPT) and siPlk1 could prohibit cancer development with combined effects. To ensure the two drugs could be simultaneously delivered to tumor region with high loading content, and the modulator siPlk1 could be released in advance to down-regulate the Plk1 expression to improve the sensitivity of CPT to cancer cells, dual sensitive and temporally controlled CPT prodrug based cationic liposomes with siPlk1 codelivery system was constructed. The pH-sensitive zwitterionic polymer poly(carboxybetaine) (PCB) was conjugated with CPT through pH and esterase-sensitive ester bond to enhance the stability and loading content of CPT. CPT-based cationic liposomes consisted of CPT-PCB prodrug and cationic lipid DDAB were then constructed for siRNA codelivery for combination therapy. The dual sensitive CPT-PCB/siPlk1 lipoplexes simultaneously delivered the two drugs to tumor cells and enabled a temporally controlled release of two drugs, that the siRNA was quickly released after 4 h incubation due to the protonation of PCB in endosomes/lysosomes, and CPT was released in a sustained manner in response to pH and esterase and highly accumulated in nucleus after 12 h incubation. The CPT-PCB/siPlk1 lipoplexes induced significant cell apoptosis and cytotoxicity in vitro with a synergistic effect. Furthermore, the dual sensitive CPT-PCB lipoplexes enhanced the tumor accumulation of the two payloads and exhibited a synergistic tumor suppression effect in tumor-bearing mice in vivo, which proved to be a promising delivery system for codelivery of CPT and siPlk1 for cancer therapy. (C) 2014 Elsevier Ltd. All rights reserved. |
| 英文摘要 | The combination of chemotherapeutic drug camptothecin (CPT) and siPlk1 could prohibit cancer development with combined effects. To ensure the two drugs could be simultaneously delivered to tumor region with high loading content, and the modulator siPlk1 could be released in advance to down-regulate the Plk1 expression to improve the sensitivity of CPT to cancer cells, dual sensitive and temporally controlled CPT prodrug based cationic liposomes with siPlk1 codelivery system was constructed. The pH-sensitive zwitterionic polymer poly(carboxybetaine) (PCB) was conjugated with CPT through pH and esterase-sensitive ester bond to enhance the stability and loading content of CPT. CPT-based cationic liposomes consisted of CPT-PCB prodrug and cationic lipid DDAB were then constructed for siRNA codelivery for combination therapy. The dual sensitive CPT-PCB/siPlk1 lipoplexes simultaneously delivered the two drugs to tumor cells and enabled a temporally controlled release of two drugs, that the siRNA was quickly released after 4 h incubation due to the protonation of PCB in endosomes/lysosomes, and CPT was released in a sustained manner in response to pH and esterase and highly accumulated in nucleus after 12 h incubation. The CPT-PCB/siPlk1 lipoplexes induced significant cell apoptosis and cytotoxicity in vitro with a synergistic effect. Furthermore, the dual sensitive CPT-PCB lipoplexes enhanced the tumor accumulation of the two payloads and exhibited a synergistic tumor suppression effect in tumor-bearing mice in vivo, which proved to be a promising delivery system for codelivery of CPT and siPlk1 for cancer therapy. (C) 2014 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Engineering, Biomedical ; Materials Science, Biomaterials |
| 研究领域[WOS] | Engineering ; Materials Science |
| 关键词[WOS] | DRUG-DELIVERY ; GENE DELIVERY ; CO-DELIVERY ; POLYMERIC MICELLES ; SYSTEMIC DELIVERY ; ENDOSOMAL ESCAPE ; CANCER-THERAPY ; DOXORUBICIN ; PH ; NANOPARTICLES |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000342890200017 |
| 公开日期 | 2014-12-01 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11677]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Zhengzhou Univ, Sch Pharmaceut Sci, Zhengzhou 450001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yan,Liu, Ruiyuan,Yang, Jun,et al. Dual sensitive and temporally controlled camptothecin prodrug liposomes codelivery of siRNA for high efficiency tumor therapy[J]. BIOMATERIALS,2014,35(36):9731-9745. |
| APA | Li, Yan,Liu, Ruiyuan,Yang, Jun,Ma, Guanghui,Zhang, Zhenzhong,&Zhang, Xin.(2014).Dual sensitive and temporally controlled camptothecin prodrug liposomes codelivery of siRNA for high efficiency tumor therapy.BIOMATERIALS,35(36),9731-9745. |
| MLA | Li, Yan,et al."Dual sensitive and temporally controlled camptothecin prodrug liposomes codelivery of siRNA for high efficiency tumor therapy".BIOMATERIALS 35.36(2014):9731-9745. |
入库方式： OAI收割
来源：过程工程研究所
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