M-cell targeted polymeric lipid nanoparticles containing a toll-like receptor agonist to boost oral immunity
文献类型：期刊论文
| 作者 | Ma, Tongtong1,2; Wang, Lianyan2; Yang, Tingyuan2; Ma, Guanghui2; Wang, Siling1 |
| 刊名 | INTERNATIONAL JOURNAL OF PHARMACEUTICS
 |
| 出版日期 | 2014-10-01 |
| 卷号 | 473期号:1-2页码:296-303 |
| 关键词 | Oral delivery Vaccine Polymeric lipid nanoparticles M-cell targeting Toll-like receptor agonist |
| ISSN号 | 0378-5173 |
| 其他题名 | Int. J. Pharm. |
| 中文摘要 | Oral delivery of antigens is patient-friendly and efficient way of treating intestinal infections. However, the efficacy of oral vaccines is limited by degradation in the gastrointestinal (GI) tract and poor absorption by enterocytes and antigen-presenting cells (APC). Here we report ulex europaeus agglutinin-1 (UEA-1) conjugated poly (D,L-lactide-co-glycolide) (PLGA)-lipid nanoparticles (NP) containing a Toll-like receptor (TLR)-agonist monophosphoryl lipid A (MPL) as an oral vaccine delivery system. The uniform-sized PLGA-lipid NPs (simplified as lipid NPs) were produced by the premix membrane emulsification method. They can protect the entrapped model antigen ovalbumin (OVA) from exposure to the GI tract and release the OVA in a controlled manner. With UEA-1 and MPL modification, the UEA-MPL/lipid NPs can be effectively transported by M-cells and captured by mucosal dendritic cells (DCs). After in vivo vaccination, the OVA-UEA-MPL/lipid NPs stimulated the most effective mucosal IgA and serum IgG antibodies during the oral formulations. These results suggest that this MPL containing M-cell targeted lipid NP can potentially be used as a universally robust oral vaccine delivery system. (C) 2014 Elsevier B.V. All rights reserved. |
| 英文摘要 | Oral delivery of antigens is patient-friendly and efficient way of treating intestinal infections. However, the efficacy of oral vaccines is limited by degradation in the gastrointestinal (GI) tract and poor absorption by enterocytes and antigen-presenting cells (APC). Here we report ulex europaeus agglutinin-1 (UEA-1) conjugated poly (D,L-lactide-co-glycolide) (PLGA)-lipid nanoparticles (NP) containing a Toll-like receptor (TLR)-agonist monophosphoryl lipid A (MPL) as an oral vaccine delivery system. The uniform-sized PLGA-lipid NPs (simplified as lipid NPs) were produced by the premix membrane emulsification method. They can protect the entrapped model antigen ovalbumin (OVA) from exposure to the GI tract and release the OVA in a controlled manner. With UEA-1 and MPL modification, the UEA-MPL/lipid NPs can be effectively transported by M-cells and captured by mucosal dendritic cells (DCs). After in vivo vaccination, the OVA-UEA-MPL/lipid NPs stimulated the most effective mucosal IgA and serum IgG antibodies during the oral formulations. These results suggest that this MPL containing M-cell targeted lipid NP can potentially be used as a universally robust oral vaccine delivery system. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Pharmacology & Pharmacy |
| 研究领域[WOS] | Pharmacology & Pharmacy |
| 关键词[WOS] | CHITOSAN NANOPARTICLES ; VACCINE DELIVERY ; DENDRITIC CELLS ; MUCOSAL ; RESPONSES ; ANTIGENS ; TRANSPORT ; BACTERIA |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000342656800033 |
| 公开日期 | 2014-12-01 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11682]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Shenyang Pharmaceut Univ, Dept Pharmaceut, Shenyang 110016, Liaoning, Peoples R China 2.Chinese Acad Sci, Natl Key Lab Biochem Engn, PM Key Lab Biopharmaceut Prod & Formulat Engn, Inst Proc Engn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Tongtong,Wang, Lianyan,Yang, Tingyuan,et al. M-cell targeted polymeric lipid nanoparticles containing a toll-like receptor agonist to boost oral immunity[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2014,473(1-2):296-303. |
| APA | Ma, Tongtong,Wang, Lianyan,Yang, Tingyuan,Ma, Guanghui,&Wang, Siling.(2014).M-cell targeted polymeric lipid nanoparticles containing a toll-like receptor agonist to boost oral immunity.INTERNATIONAL JOURNAL OF PHARMACEUTICS,473(1-2),296-303. |
| MLA | Ma, Tongtong,et al."M-cell targeted polymeric lipid nanoparticles containing a toll-like receptor agonist to boost oral immunity".INTERNATIONAL JOURNAL OF PHARMACEUTICS 473.1-2(2014):296-303. |
入库方式： OAI收割
来源：过程工程研究所
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