Biodegradable polylactide microspheres enhance specific immune response induced by Hepatitis B surface antigen
文献类型：期刊论文
| 作者 | Qiu, Shaohui1; Wei, Qiang2; Liang, Zhenglun1; Ma, Guanghui2; Wang, Lianyan2; An, Wenqi3; Ma, Xiaowei3; Fang, Xin1; He, Peng1; Li, Hemin1 |
| 刊名 | HUMAN VACCINES & IMMUNOTHERAPEUTICS
 |
| 出版日期 | 2014 |
| 卷号 | 10期号:8页码:2350-2356 |
| 关键词 | polylactide microspheres HBsAg IFN-gamma CTL anti-HBs |
| ISSN号 | 2164-5515 |
| 其他题名 | Human Vaccines Immunother. |
| 中文摘要 | Hepatitis B (HB) infection caused by Hepatitis B virus (HBV) is the most common liver disease in the world. HB vaccine, when administered in conjunction with alum adjuvants, induces Th2 immunity that confers protection against HBV. However, currently available vaccine formulations and adjuvants do not elicit adequate Th1 and CTL responses that are important for prevention of maternal transmission of the virus. Microspheres synthesized from poly (D, L-lactide-co-glycolide) (PLGA) or poly (D, L-lactide) (PLA) polymers have been considered as promising tools for in vivo delivery of antigens and drugs. Here we describe PLA microspheres synthesized by premix membrane emulsification method and their application in formulating a new microsphere based HB vaccine. To evaluate the immunogenicity of this microsphere vaccine, BALB/c mice were immunized with microsphere vaccine and a series of immunological assays were conducted. Results of Enzyme-linked ImmunoSpot (ELISPOT) assays revealed that the number of interferon-gamma (IFN-gamma)-producing splenocytes and CD8(+) T cells increased significantly in the microsphere vaccine group. Microsphere vaccine group showed enhanced specific cell lysis when compared with HB surface antigen (HBsAg) only group in Cr-51 cytotoxicity assays. Moreover, microsphere vaccine elicited a comparable level of antibody production as that of HB vaccine administered with alum adjuvant. We show that phagocytosis of HBsAg by dendritic cells is more pronounced in microsphere vaccine group when compared with other control groups. These results clearly demonstrate the potential of using PLA microspheres as effective HB vaccine adjuvants for an enhanced Th1 immune response. |
| 英文摘要 | Hepatitis B (HB) infection caused by Hepatitis B virus (HBV) is the most common liver disease in the world. HB vaccine, when administered in conjunction with alum adjuvants, induces Th2 immunity that confers protection against HBV. However, currently available vaccine formulations and adjuvants do not elicit adequate Th1 and CTL responses that are important for prevention of maternal transmission of the virus. Microspheres synthesized from poly (D, L-lactide-co-glycolide) (PLGA) or poly (D, L-lactide) (PLA) polymers have been considered as promising tools for in vivo delivery of antigens and drugs. Here we describe PLA microspheres synthesized by premix membrane emulsification method and their application in formulating a new microsphere based HB vaccine. To evaluate the immunogenicity of this microsphere vaccine, BALB/c mice were immunized with microsphere vaccine and a series of immunological assays were conducted. Results of Enzyme-linked ImmunoSpot (ELISPOT) assays revealed that the number of interferon-gamma (IFN-gamma)-producing splenocytes and CD8(+) T cells increased significantly in the microsphere vaccine group. Microsphere vaccine group showed enhanced specific cell lysis when compared with HB surface antigen (HBsAg) only group in Cr-51 cytotoxicity assays. Moreover, microsphere vaccine elicited a comparable level of antibody production as that of HB vaccine administered with alum adjuvant. We show that phagocytosis of HBsAg by dendritic cells is more pronounced in microsphere vaccine group when compared with other control groups. These results clearly demonstrate the potential of using PLA microspheres as effective HB vaccine adjuvants for an enhanced Th1 immune response. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Immunology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Immunology |
| 关键词[WOS] | VIRUS ; MICROPARTICLES ; RELEASE ; VACCINE ; IMMUNOGENICITY ; NANOPARTICLES ; IMMUNIZATION ; PATHOGENESIS ; INDUCTION ; INFECTION |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000344318300034 |
| 公开日期 | 2014-12-01 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11684]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Minist Hlth Res Qual & Standardizat Biotech Prod, Key Lab, Natl Inst Food & Drug Control, Div Hepatitis Virus Vaccine, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China 3.Hualan Biol Engn Inc Xinxiang, Xinxiang, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, Shaohui,Wei, Qiang,Liang, Zhenglun,et al. Biodegradable polylactide microspheres enhance specific immune response induced by Hepatitis B surface antigen[J]. HUMAN VACCINES & IMMUNOTHERAPEUTICS,2014,10(8):2350-2356. |
| APA | Qiu, Shaohui.,Wei, Qiang.,Liang, Zhenglun.,Ma, Guanghui.,Wang, Lianyan.,...&Hu, Zhongyu.(2014).Biodegradable polylactide microspheres enhance specific immune response induced by Hepatitis B surface antigen.HUMAN VACCINES & IMMUNOTHERAPEUTICS,10(8),2350-2356. |
| MLA | Qiu, Shaohui,et al."Biodegradable polylactide microspheres enhance specific immune response induced by Hepatitis B surface antigen".HUMAN VACCINES & IMMUNOTHERAPEUTICS 10.8(2014):2350-2356. |
入库方式： OAI收割
来源：过程工程研究所
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