SERS Imaging of Cell-Surface Biomolecules Metabolically Labeled with Bioorthogonal Raman Reporters
文献类型：期刊论文
| 作者 | Xiao, Ming1; Lin, Liang1; Li, Zefan1; Liu, Jie2; Hong, Senlian1; Li, Yaya1; Zheng, Meiling2; Duan, Xuanming2; Chen, Xing1 |
| 刊名 | CHEMISTRY-AN ASIAN JOURNAL
 |
| 出版日期 | 2014-08-01 |
| 卷号 | 9期号:8页码:2040-2044 |
| 关键词 | biomolecules bioorthogonality cell surface Raman imaging SERS imaging |
| ISSN号 | 1861-4728 |
| 其他题名 | Chem.-Asian J. |
| 中文摘要 | Live imaging of biomolecules with high specificity and sensitivity as well as minimal perturbation is essential for studying cellular processes. Here, we report the development of a bioorthogonal surface-enhanced Raman scattering (SERS) imaging approach that exploits small Raman reporters for visualizing cell-surface biomolecules. The cells were cultured and imaged by SERS microscopy on arrays of Raman-enhancing nanoparticles coated on silicon wafers or glass slides. The Raman reporters including azides, alkynes, and carbon-deuterium bonds are small in size and spectroscopically bioorthogonal (background-free). We demonstrated that various cell-surface biomolecules including proteins, glycans, and lipids were metabolically incorporated with the corresponding precursors bearing a Raman reporter and visualized by SERS microscopy. The coupling of SERS microscopy with bioorthogonal Raman reporters expands the capabilities of live-cell microscopy beyond the modalities of fluorescence and label-free imaging. |
| 英文摘要 | Live imaging of biomolecules with high specificity and sensitivity as well as minimal perturbation is essential for studying cellular processes. Here, we report the development of a bioorthogonal surface-enhanced Raman scattering (SERS) imaging approach that exploits small Raman reporters for visualizing cell-surface biomolecules. The cells were cultured and imaged by SERS microscopy on arrays of Raman-enhancing nanoparticles coated on silicon wafers or glass slides. The Raman reporters including azides, alkynes, and carbon-deuterium bonds are small in size and spectroscopically bioorthogonal (background-free). We demonstrated that various cell-surface biomolecules including proteins, glycans, and lipids were metabolically incorporated with the corresponding precursors bearing a Raman reporter and visualized by SERS microscopy. The coupling of SERS microscopy with bioorthogonal Raman reporters expands the capabilities of live-cell microscopy beyond the modalities of fluorescence and label-free imaging. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | SELF-ASSEMBLED MONOLAYERS ; NONCANONICAL AMINO-ACIDS ; IN-VIVO ; FLUORESCENCE MICROSCOPY ; SCATTERING ; PHOSPHOLIPIDS ; NANOPARTICLES ; SPECTROSCOPY ; CHEMISTRY ; DISCOVERY |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000342675900007 |
| 公开日期 | 2014-12-01 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11690]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Peking Univ, Peking Tsinghua Ctr Life Sci, Synthet & Funct Biomol Ctr, Coll Chem & Mol Engn,Dept Chem Biol,Beijing Natl, Beijing 100871, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, Ming,Lin, Liang,Li, Zefan,et al. SERS Imaging of Cell-Surface Biomolecules Metabolically Labeled with Bioorthogonal Raman Reporters[J]. CHEMISTRY-AN ASIAN JOURNAL,2014,9(8):2040-2044. |
| APA | Xiao, Ming.,Lin, Liang.,Li, Zefan.,Liu, Jie.,Hong, Senlian.,...&Chen, Xing.(2014).SERS Imaging of Cell-Surface Biomolecules Metabolically Labeled with Bioorthogonal Raman Reporters.CHEMISTRY-AN ASIAN JOURNAL,9(8),2040-2044. |
| MLA | Xiao, Ming,et al."SERS Imaging of Cell-Surface Biomolecules Metabolically Labeled with Bioorthogonal Raman Reporters".CHEMISTRY-AN ASIAN JOURNAL 9.8(2014):2040-2044. |
入库方式： OAI收割
来源：过程工程研究所
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