中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Polycation-decorated PLA microspheres induce robust immune responses via commonly used parenteral administration routes

文献类型:期刊论文

作者Chen, Xiaoming1,2; Wang, Lianyan1; Liu, Qi1,2; Jia, Jilei1,2; Liu, Yuan1,2; Zhang, Weifeng1,2; Ma, Guanghui1; Su, Zhiguo1
刊名INTERNATIONAL IMMUNOPHARMACOLOGY
出版日期2014-12-01
卷号23期号:2页码:592-602
关键词Administration route Cationic microsphere Alum Antibodies Cytokines
ISSN号1567-5769
其他题名Int. Immunopharmacol.
中文摘要Recombinant viral subunit-based vaccines have gained increasing attention due to their enhanced safety over the classic live-attenuated or inactivated vaccines. The low immunogenicity of the subunit antigen alone, however, requires the addition of an adjuvant to induce immunity. Particulate-based delivery systems have great potential for developing new vaccine adjuvants, compared to traditional aluminum-based saline adjuvants. The physicochemical properties of particulate vaccines have been extensively investigated; however, few studies have focused on how the administration route of various adjuvant-antigen combinations impacts the efficacy of the immune response. Here, for the first time, the viral Hepatitis B surface antigen (HBsAg) was combined with aluminum-based or cationic-microsphere (MP) based adjuvants to investigate the characteristics of immune responses elicited after immunization via the subcutaneous, intramuscular, or intraperitoneal routes respectively. In vitro, the MP-based vaccine significantly increased dendritic cell (DC) activation with up-regulated CD40 and CD80 expression and IL-12 production compared to alum-based vaccine. After immunization, both MP and alum-based vaccines produced increased IgG titers in mice. The administration route of these vaccines did influenced immune responses. The MP-based vaccine delivered via the intramuscular route yielded the highest levels of the IgG2a isotype. The alum-based vaccine, delivered via the same route, produced an IgG1-dominated humoral immune response. Moreover, subcutaneous and intramuscular immunizations with MP-based vaccine augmented Granzyme B, Th1-type cytokines (IL-2, IL-12, and IFN-gamma), and Th2 cytokine IL-4 secretions. These results demonstrate that MP-based vaccines have the capacity to induce higher cellular and humoral immune response especially via an intramuscular administration route than an alum-based vaccine. (C) 2014 Elsevier B.V. All rights reserved.
英文摘要Recombinant viral subunit-based vaccines have gained increasing attention due to their enhanced safety over the classic live-attenuated or inactivated vaccines. The low immunogenicity of the subunit antigen alone, however, requires the addition of an adjuvant to induce immunity. Particulate-based delivery systems have great potential for developing new vaccine adjuvants, compared to traditional aluminum-based saline adjuvants. The physicochemical properties of particulate vaccines have been extensively investigated; however, few studies have focused on how the administration route of various adjuvant-antigen combinations impacts the efficacy of the immune response. Here, for the first time, the viral Hepatitis B surface antigen (HBsAg) was combined with aluminum-based or cationic-microsphere (MP) based adjuvants to investigate the characteristics of immune responses elicited after immunization via the subcutaneous, intramuscular, or intraperitoneal routes respectively. In vitro, the MP-based vaccine significantly increased dendritic cell (DC) activation with up-regulated CD40 and CD80 expression and IL-12 production compared to alum-based vaccine. After immunization, both MP and alum-based vaccines produced increased IgG titers in mice. The administration route of these vaccines did influenced immune responses. The MP-based vaccine delivered via the intramuscular route yielded the highest levels of the IgG2a isotype. The alum-based vaccine, delivered via the same route, produced an IgG1-dominated humoral immune response. Moreover, subcutaneous and intramuscular immunizations with MP-based vaccine augmented Granzyme B, Th1-type cytokines (IL-2, IL-12, and IFN-gamma), and Th2 cytokine IL-4 secretions. These results demonstrate that MP-based vaccines have the capacity to induce higher cellular and humoral immune response especially via an intramuscular administration route than an alum-based vaccine. (C) 2014 Elsevier B.V. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Immunology ; Pharmacology & Pharmacy
研究领域[WOS]Immunology ; Pharmacology & Pharmacy
关键词[WOS]HEPATITIS-B-VACCINE ; CD8(+) T-CELLS ; DENDRITIC CELLS ; DELIVERY-SYSTEMS ; ANTIGEN-DELIVERY ; ANTIBODY-RESPONSE ; CUTTING EDGE ; DNA VACCINE ; NANOPARTICLES ; ADJUVANT
收录类别SCI
原文出处://WOS:000346954000031
语种英语
WOS记录号WOS:000346954000031
公开日期2015-04-01
源URL[http://ir.ipe.ac.cn/handle/122111/11712]  
专题过程工程研究所_研究所(批量导入)
作者单位1.Chinese Acad Sci, Inst Proc Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Graduated Univ, Beijing 100049, Peoples R China
推荐引用方式
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Chen, Xiaoming,Wang, Lianyan,Liu, Qi,et al. Polycation-decorated PLA microspheres induce robust immune responses via commonly used parenteral administration routes[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2014,23(2):592-602.
APA Chen, Xiaoming.,Wang, Lianyan.,Liu, Qi.,Jia, Jilei.,Liu, Yuan.,...&Su, Zhiguo.(2014).Polycation-decorated PLA microspheres induce robust immune responses via commonly used parenteral administration routes.INTERNATIONAL IMMUNOPHARMACOLOGY,23(2),592-602.
MLA Chen, Xiaoming,et al."Polycation-decorated PLA microspheres induce robust immune responses via commonly used parenteral administration routes".INTERNATIONAL IMMUNOPHARMACOLOGY 23.2(2014):592-602.

入库方式: OAI收割

来源:过程工程研究所

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