Formation of Nitrile Hydratase Cross-Linked Enzyme Aggregates in Mesoporous Onion-like Silica: Preparation and Catalytic Properties
文献类型：期刊论文
| 作者 | Gao, J.; Wang, Q.; Jiang, Y. J.; Gao, J. K.; Liu, Z. H.; Zhou, L. Y.; Zhang, Y. F. |
| 刊名 | Industrial & Engineering Chemistry Research
 |
| 出版日期 | 2015 |
| 期号 | 1页码:83-90 |
| 关键词 | ALPHA-AMYLASE IMMOBILIZATION CLEAS STABILIZATION BIOCATALYSIS LACCASE DECOLORIZATION BIOCONVERSION ACRYLAMIDE LINKING |
| 其他题名 | Ind. Eng. Chem. Res. |
| 中文摘要 | Nitrile hydratase cross-linked enzyme aggregates (CLEAs) were formed in mesoporous onion-like silica (NHase-CLEAs@MOS) by using macromolecular dextran polyaldehyde as a cross-linker through the carrier-bound CLEAs method. The effect of preparation parameters on the recovery of enzyme activity was investigated. The properties such as pH, thermal and storage stability, and kinetic parameters of NHase-CLEAs@MOS were also studied. The maximum amount of NHase absorbed in MOS was 535 mg/g. Under optimized conditions, the maximum activity recovery of NHase-CLEAs@MOS was 48.2%. The stabilities of NHase-CLEAs@MOS were improved significantly compared to the NHase@MOS prepared by physical adsorption and free NHase. This work demonstrated that the mesoporous onion-like silica can be efficiently employed as host materials for NHase immobilization, and the carrier-bound CLEAs method can lead to enhanced activity and stability of the immobilized enzymes. |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| 公开日期 | 2015-04-01 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11731]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | Gao, J.,Wang, Q.,Jiang, Y. J.,et al. Formation of Nitrile Hydratase Cross-Linked Enzyme Aggregates in Mesoporous Onion-like Silica: Preparation and Catalytic Properties[J]. Industrial & Engineering Chemistry Research,2015(1):83-90. |
| APA | Gao, J..,Wang, Q..,Jiang, Y. J..,Gao, J. K..,Liu, Z. H..,...&Zhang, Y. F..(2015).Formation of Nitrile Hydratase Cross-Linked Enzyme Aggregates in Mesoporous Onion-like Silica: Preparation and Catalytic Properties.Industrial & Engineering Chemistry Research(1),83-90. |
| MLA | Gao, J.,et al."Formation of Nitrile Hydratase Cross-Linked Enzyme Aggregates in Mesoporous Onion-like Silica: Preparation and Catalytic Properties".Industrial & Engineering Chemistry Research .1(2015):83-90. |
入库方式： OAI收割
来源：过程工程研究所
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。