Polyelectrolyte Doped Hollow Nanofibers for Positional Assembly of Bienzyme System for Cascade Reaction at O/W Interface
文献类型：期刊论文
| 作者 | Ji, Xiaoyuan1,2; Su, Zhiguo1,5; Wang, Ping1,3,4; Ma, Guanghui1; Zhang, Songping1,5 |
| 刊名 | ACS CATALYSIS
 |
| 出版日期 | 2014-12-01 |
| 卷号 | 4期号:12页码:4548-4559 |
| 关键词 | cascade reactions coaxial electrospinning polyelectrolyte hollow nanofibers positional assembly |
| ISSN号 | 2155-5435 |
| 其他题名 | ACS Catal. |
| 中文摘要 | Cationic polyelectrolyte doped hollow nanofibers prepared via facial coaxial electrospinning technology have been used for positional assembly of two enzymes, glucose oxidase (GOD) and Candida antactica lipase B (CALB), at two different positions, namely, in their lumen and on their surface. Therefore, the result is four combinations, including lumen (GOD+CALB), surface (GOD+CALB), surface (GOD)-lumen (CALB), and lumen (GOD)-surface (CALB). Surface attachment of enzymes was achieved by layer-by-layer (LbL) technology, which is based on the ion-exchange interactions between oppositely charged enzymes and polyelectrolyte that was doped in hollow nanofibers; whereas placing enzymes inside the lumen of hollow nanofibers was realized by in situ encapsulation during coelectrospinning. The hollow nanofibers-based biencyine systems were used for a cascade reaction in an oil-aqueous biphasic system, in which glucose was oxidized by GOD to generate H2O2, which was used as substrate and oxidant for CALB-catalyzed epoxidation of oleic acid in the second step. The bienzyme nanofibers membrane was found to float spontaneously at the O/W interface, which is advantageous to biphasic biocatalysis. Assembly strategies of the two enzymes affect their biocatalytic efficiency significantly by influencing the utilization efficiency of H2O2 in the reaction process. The highest reaction rate was attained by lumen (GOD)-surface (CALB), corresponding to 114.45 times enhancement as compared to that of the free bienzyme system. |
| 英文摘要 | Cationic polyelectrolyte doped hollow nanofibers prepared via facial coaxial electrospinning technology have been used for positional assembly of two enzymes, glucose oxidase (GOD) and Candida antactica lipase B (CALB), at two different positions, namely, in their lumen and on their surface. Therefore, the result is four combinations, including lumen (GOD+CALB), surface (GOD+CALB), surface (GOD)-lumen (CALB), and lumen (GOD)-surface (CALB). Surface attachment of enzymes was achieved by layer-by-layer (LbL) technology, which is based on the ion-exchange interactions between oppositely charged enzymes and polyelectrolyte that was doped in hollow nanofibers; whereas placing enzymes inside the lumen of hollow nanofibers was realized by in situ encapsulation during coelectrospinning. The hollow nanofibers-based biencyine systems were used for a cascade reaction in an oil-aqueous biphasic system, in which glucose was oxidized by GOD to generate H2O2, which was used as substrate and oxidant for CALB-catalyzed epoxidation of oleic acid in the second step. The bienzyme nanofibers membrane was found to float spontaneously at the O/W interface, which is advantageous to biphasic biocatalysis. Assembly strategies of the two enzymes affect their biocatalytic efficiency significantly by influencing the utilization efficiency of H2O2 in the reaction process. The highest reaction rate was attained by lumen (GOD)-surface (CALB), corresponding to 114.45 times enhancement as compared to that of the free bienzyme system. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Physical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | UNSATURATED FATTY-ACID ; CHEMOENZYMATIC EPOXIDATION ; BINDING CHLOROPEROXIDASE ; ENZYME IMMOBILIZATION ; ARTIFICIAL CELLS ; BRADFORD METHOD ; SOYBEAN OIL ; PLANT OILS ; BIOCATALYSIS ; PERFORMANCE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000346041600035 |
| 公开日期 | 2015-04-01 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11754]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA 4.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA 5.Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Xiaoyuan,Su, Zhiguo,Wang, Ping,et al. Polyelectrolyte Doped Hollow Nanofibers for Positional Assembly of Bienzyme System for Cascade Reaction at O/W Interface[J]. ACS CATALYSIS,2014,4(12):4548-4559. |
| APA | Ji, Xiaoyuan,Su, Zhiguo,Wang, Ping,Ma, Guanghui,&Zhang, Songping.(2014).Polyelectrolyte Doped Hollow Nanofibers for Positional Assembly of Bienzyme System for Cascade Reaction at O/W Interface.ACS CATALYSIS,4(12),4548-4559. |
| MLA | Ji, Xiaoyuan,et al."Polyelectrolyte Doped Hollow Nanofibers for Positional Assembly of Bienzyme System for Cascade Reaction at O/W Interface".ACS CATALYSIS 4.12(2014):4548-4559. |
入库方式： OAI收割
来源：过程工程研究所
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