Facile immobilization of enzyme on three dimensionally ordered macroporous silica via a biomimetic coating
文献类型：期刊论文
| 作者 | Jiang, Yanjun1; Wang, Yaping1; Wang, Hua1; Zhou, Liya1; Gao, Jing1; Zhang, Yufei2; Zhang, Xu1; Wang, Xiaomei1; Li, Jian3 |
| 刊名 | NEW JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2015 |
| 卷号 | 39期号:2页码:978-984 |
| 关键词 | PENICILLIN-G ACYLASE MESOSTRUCTURED CELLULAR FOAMS MESOPOROUS SILICA CROSS-LINKING BIOCATALYSTS IMPROVEMENT STABILITY LIPASE |
| ISSN号 | 1144-0546 |
| 其他题名 | New J. Chem. |
| 中文摘要 | This study describes a general strategy using norepinephrine (NE) as a stable anchor for the immobilization of enzyme onto three-dimensionally ordered macroporous (3DOM) silica. Penicillin G acylase (PGA) was chosen as a model enzyme. The effect of pH and temperature on the activity of PGA@PN-SiO2 (PGA immobilized on poly(norepinephrine)-modified 3DOM silica) was investigated. The operational stability, storage stability and kinetic properties of the PGA@PN-SiO2 were also examined. Compared with free PGA and PGA@SiO2 (PGA immobilized on blank 3DOM silica through physical adsorption), the stabilities of PGA@PN-SiO2 were improved significantly. PGA@PN-SiO2 retained high activity during the hydrolysis in a continuous packed-bed reactor (PBR) after 30 catalytic cycles, which demonstrated that PGA@PN-SiO2 can bear endurance of continuous catalysis. The method presented in this study has broad potential for immobilizing enzymes and other biomolecules. |
| 英文摘要 | This study describes a general strategy using norepinephrine (NE) as a stable anchor for the immobilization of enzyme onto three-dimensionally ordered macroporous (3DOM) silica. Penicillin G acylase (PGA) was chosen as a model enzyme. The effect of pH and temperature on the activity of PGA@PN-SiO2 (PGA immobilized on poly(norepinephrine)-modified 3DOM silica) was investigated. The operational stability, storage stability and kinetic properties of the PGA@PN-SiO2 were also examined. Compared with free PGA and PGA@SiO2 (PGA immobilized on blank 3DOM silica through physical adsorption), the stabilities of PGA@PN-SiO2 were improved significantly. PGA@PN-SiO2 retained high activity during the hydrolysis in a continuous packed-bed reactor (PBR) after 30 catalytic cycles, which demonstrated that PGA@PN-SiO2 can bear endurance of continuous catalysis. The method presented in this study has broad potential for immobilizing enzymes and other biomolecules. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | PENICILLIN-G ACYLASE ; MESOSTRUCTURED CELLULAR FOAMS ; MESOPOROUS SILICA ; CROSS-LINKING ; BIOCATALYSTS ; IMPROVEMENT ; STABILITY ; LIPASE |
| 收录类别 | SCI |
| 原文出处 | |
| 语种 | 英语 |
| WOS记录号 | WOS:000349222800026 |
| 公开日期 | 2015-04-01 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/11761]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 作者单位 | 1.Hebei Univ Technol, Sch Chem Engn & Technol, Tianjin 300130, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Tianjin Univ Sci & Technolgy, Coll Mat Sci & Chem Engn, Tianjin 300457, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Yanjun,Wang, Yaping,Wang, Hua,et al. Facile immobilization of enzyme on three dimensionally ordered macroporous silica via a biomimetic coating[J]. NEW JOURNAL OF CHEMISTRY,2015,39(2):978-984. |
| APA | Jiang, Yanjun.,Wang, Yaping.,Wang, Hua.,Zhou, Liya.,Gao, Jing.,...&Li, Jian.(2015).Facile immobilization of enzyme on three dimensionally ordered macroporous silica via a biomimetic coating.NEW JOURNAL OF CHEMISTRY,39(2),978-984. |
| MLA | Jiang, Yanjun,et al."Facile immobilization of enzyme on three dimensionally ordered macroporous silica via a biomimetic coating".NEW JOURNAL OF CHEMISTRY 39.2(2015):978-984. |
入库方式： OAI收割
来源：过程工程研究所
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